薄层荧光扫描法测定吴茱萸药材及其制剂中5种生物碱的含量

目的：用薄层荧光扫描法测定吴茱萸及加味左金丸中5种生物碱:羟基吴茱萸碱(hydroxyevodiamine, I)、N-甲酰二氢吴茱萸次碱(N-formyldihydrorutaecarpine, II)、吴茱萸酰胺甲(goshuyuamide-I, III)、吴茱萸碱（evodiamine, IV)及吴茱萸次碱(rutaecarpine， V)。方法：在高效硅胶薄层板上点样后，二次展开。展开剂:（1）氯仿—石油醚—甲醇—环己烷（5∶3∶0.4∶0.4）；(2)氯仿—甲醇(4.1∶0.3)。展开后用浓盐酸衍生化，荧光扫描测定。结果：各生物碱的线性范围在20.5～245 ng，回收率为96.8%～104.5%，并用此法测定了不同产地吴茱萸及不同批号的加味左金丸中生物碱的含量。结论：方法灵敏度高、样品用量少，可为寻找和利用植物资源，控制生药及方剂质量提供科学依据。

DETERMINATION OF FIVE ALKALOIDS OF EVODIA RUTAECARPA AND ITS PREPARATION BY HPTLC FLUORESCENT SCANNING

AIM: To adopt a TLC fluorescent scanning method for the separation and determination of the alkaloids: hydroxyevodiamine (I), Nformyldihydrorutaecarpine (II), goshuyuamideI (III), evodiamine (IV) and rutaecarpine (V) in Evodia rutaecarpa and its preparation. METHODS: Chloroformpetroleum ethermethanolcyclohexane (530404) was used as developing solvent on the HPTLC silica gel plate for first development; and chloroformmethanol (4103) for the second development. After that, the spots were derivatized by concentrated hydrochloric acid and then determined with a Shimadzu CS930 TLC scanner. RESULTS: The calibration curves were linear in the range of 205245 ng for the five alkaloids and the recoveries were 96845%. The method has been applied to the analysis of various samples and their preparations. CONCLUSION: This highly sensitive method required small amount of sample. It offered a scientific basis for seeking and utilizing botanic resources, and controlling the quality of herb medicines and preparations.