| 人载脂蛋白E的PCR－RFLP基因分型 |
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| 引用本文： | 游学科,赵水平,朱铁兵,王钟林. 人载脂蛋白E的PCR－RFLP基因分型[J]. 中南大学学报(医学版), 1995, 0(4) |
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| 作者姓名： | 游学科 赵水平 朱铁兵 王钟林 |
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| 作者单位： | You Xueke Central laboratory，The Second Affiliated Hospijal，Hunan Medical University Zhao Shuiping; Zhu Tiebin ;Wang Zhongling Department of Cardiology，The Second Affiliated Hospital，Hunan Medical University |
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| 摘 要： | ![]()
采用两对引物进行套式聚合酶链式反应（ＰＣＲ），扩增人载脂蛋白Ｅ（ａｐｏＥ）基因第４号外显子的２３３ｂｐ特异性片段。用限制性核酸内切酶ｎｈａｌ酶解ＰＣＲ产物。聚丙烯酰胺凝胶电泳分离后进行ＰＣＲ－ＲＦＬＰ基因分型。此方法简便、灵敏，结果可靠。分析了１００名正常人ａｐｏＥ的基因型，其等位基因ｅ２，ｅ３，ｅ４频率分别为０．０５，０．８８和０．０７。
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| 关 键 词： | 脱辅基脂蛋白E类；基因；多聚酶链反应；限制性片段长度多态性分析 |
A PCR-RFLP GENE TYPING OF HUMAN APOLIPOPROTEIN E |
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| You Xueke Central laboratory,The Second Affiliated Hospijal,Hunan Medical University Zhao Shuiping, Zhu Tiebin ,Wang Zhongling. A PCR-RFLP GENE TYPING OF HUMAN APOLIPOPROTEIN E[J]. Journal of Central South University. Medical sciences, 1995, 0(4) |
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| Authors: | You Xueke Central laboratory The Second Affiliated Hospijal Hunan Medical University Zhao Shuiping Zhu Tiebin Wang Zhongling |
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| Affiliation: | You Xueke Central laboratory,The Second Affiliated Hospijal,Hunan Medical University Zhao Shuiping, Zhu Tiebin ,Wang Zhongling Department of Cardiology,The Second Affiliated Hospital,Hunan Medical University |
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| Abstract: | ![]()
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| Keywords: | apolioporotein E gene polymerase chain raction restriction fregament length polymorphism |
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