血清乙型肝炎病毒表面抗原检测弱反应结果的确认方法及其评价

目的探讨血清乙型肝炎病毒表面抗原(HBsAg)检测弱反应结果确认方法。方法34例HBsAg弱反应结果血清标本,用双份复检、中和试验和随访检测,对HBsAg结果进行确认。结果34例HBsAg平均水平(S/N)为4.28(2.12~8.07);双份复检HBsAg全部阳性;中和试验阳性31例(91.2%),阴性3例(8.80%);2次随访HBsAg均阳性30例(88.2%),均阴性4例(11.8%);中和试验和随访结果均阳性28例(82.4%),均阴性1例(2.94%),另5例中和试验和随访结果不符。结论HBsAg弱反应结果大部分可确认HBsAg阳性,几类确认方法对于明确诊断有一定价值。

Evaluation of methods for confirming the weak-reactive results of HBsAg in serum

Objective To investigate the methods for confirming the weak-reactive results of HBsAg in serum. Methods 34 serum samples were tested for HBsAg with microparticle enzyme immunoassay,weak reactive results were confirmed by different methods as duplicate test,neutralizing confirmatory test and two times follow-up test. Results The mean of HBsAg(S/N) in these serum samples was 4.28(range from(2.12) to 8.07).All of these 34 cases were positive in duplicate test.31(91.2%) were positive and 3(8.80%) were negative in neutralizing confirmatory test.30(88.2%) were positive and 4(11.8%) were negative in two times follow-up test.28(82.4%) were both positive in neutralizing confirmatory test and in two times followup test,while 1(2.94%) was both negative.In the other 5 cases,the results of neutralizing confirmatory test did not agree with two times follow-up test. Conclusions Most HBsAg weak-reactive positive results can be confirmed as true positive.These confirmatory methods are helpful in clinical diagnosis.