四君子汤对D-半乳糖衰老模型小鼠心、肝、脑组织MDA含量及端粒酶活性的影响

目的 研究四君子汤(SJ)对D-半乳糖(D-gal)衰老模型小鼠心、肝、脑组织丙二醛(MDA)含量及端粒酶活性的影响。方法 60只小鼠分为5组，除正常对照组外其余4组采用10％的D-gal0．01g／g注射于小鼠颈背部皮下，每日1次，连续6周，建立小鼠衰老模型；于造模同时，3个给药组(低、中、高剂量)分别按6g／(b·d)、12g／(kg·d)、24g／(kg·d)的药量(容积为0．2m1／10g体重)喂饲四君子汤，连续用药6周；正常对照组和模型对照组喂饲同体积的蒸馏水。TBA比色法检测小鼠心、肝、脑组织MDA含量；PCR-ELISA法检测小鼠心、肝、脑组织端粒酶活性。结果 与正常对照组相比，模型对照组小鼠心、肝、脑组织MDA含量明显增加(P<0．01)，心、肝脏组织端粒酶活性明显降低(P<0．01)，脑组织端粒酶活性无明显变化(P>0．05)；与模型对照组比较，3个给药组小鼠心、肝、脑MDA含量明显减少(P<0．01)，低、中剂量给药组小鼠心脏组织端粒酶活性有升高的趋势，但差异不显著(P>0．05)，高剂量给药组小鼠心脏组织端粒酶活性明显升高(P<0．05)；3个给药组小鼠肝组织端粒酶活性均无明显变化(P>0．05)；低、中剂量给药组小鼠脑组织端粒酶活性无明显变化(P>0．05)，高剂量给药组小鼠脑组织端粒酶活性明显升高(P<0．01)。结论 四君子汤能拮抗自由基损伤，减少D-gal衰老模型小鼠心、肝、脑组织MDA含量，并能提高其心、脑组织端粒酶活性，但对肝组织端粒酶活性无影响。

Effect of Sijunzi Decoction on Malondialdehyde Content and Telomerase Activity in Heart, Liver and Brain Tissues of D-galactose Induced Aging Model Mice

ObjectiveTo investigate the effect of Sijunzi decoction (SJZD) on malondialdehyde (MDA) content and telomerase activity in heart, liver and brain tissues of D-galactose (D-gal) induced aging model mice. MethodsD-gal aging mice model was established by cervicodorsal region subcutaneous injection with 10% D-gal once a day for six successive months. The model mice in the low-, middle- and high-dose SJZD treated groups were treated with SJZD in a dose of 6 g/kg, 12 g/kg, 24 g/kg per day respectively in the volume of 0.2 ml/10 g for 6 successive weeks. While the mice in the normal control group (NCG, non-modeled) and the model control group (MCG, modeled but untreated) were treated with distilled water instead. The MDA content and telomerase activity in heart, liver and brain tissues of mice was measured with TBA colorimetric method and PCR-ELISA respectively. ResultsIn MCG, the MDA content in heart, liver and brain tissues was significantly higher (P<0.01), and the telomerase activity in liver and heart tissues was significantly lower (P<0.01) but that in brain tissue was insignificant different to that in NCG (P>0.05) respectively. As compared with MCG, the MDA content was significantly lower in the three SJZD treated group (P<0.01). In comparison of telomerase activity between MCG and SJZD treated groups, it was shown that in heart tissue, there was an increased trend of the activity in the low-dose and middle-dose group, but with statistical insignificance (P>0.05), but it did show a significant increase in the high-dose group (P<0.05); in liver tissue no significant difference was shown between the three SJZD treated groups and MCG (P>0.05); as for that in brain tissue, significant increase only shown in the high-dose group (P<0.01). ConclusionSJZD can antagonize free radical injury, decrease the MDA content of heart, liver and brain in D-gal aging mice, and increase the telomerase activity in heart and brain tissues but with no effect on that in liver tissue.