| 结直肠癌和息肉中Caspase通路上基因表达水平 |
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| 引用本文： | 潘益峰,张明五,金明娟,张善春,陈坤,李其龙,马新源,姚开颜,余运贤.结直肠癌和息肉中Caspase通路上基因表达水平[J].浙江大学学报(医学版),2011,40(3). |
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| 作者姓名： | 潘益峰 张明五 金明娟 张善春 陈坤 李其龙 马新源 姚开颜 余运贤 |
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| 作者单位： | 1. 浙江大学公共卫生学院流行病学与卫生统计学系,浙江杭州,310058
2. 浙江省嘉善县肿瘤防治所,浙江嘉善,314100 |
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| 基金项目： | 国家自然科学基金，浙江省教育厅科研项目 |
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| 摘 要： | 目的:检测结直肠正常黏膜-结直肠息肉-结直肠癌中Caspase凋亡通路上相关基因的mRNA表达水平,探讨其在结直肠癌发生、发展中的作用.方法:收集了19例结直肠癌患者的肿瘤组织、86例结直肠息肉患者的息肉组织和10例正常对照的结直肠黏膜组织.采用实时荧光定量逆转录聚合酶链反应(RT.PCR)测定结直肠正常黏膜、结直肠息肉、结直肠癌中Caspase通路上基因(Caspase-2、-3、-6、-7、-8、-9和.10)的mRNA表达水平.结果:年龄、性别等人口学特征在正常对照、息肉及结直肠癌病例组问分布均衡;与正常黏膜组比较,除Caspase-3在结直肠癌中表达升高外,其它基因mRNA表达均下降,但都没有达到统计学上的显著性水平;此外,除Caspase-9外,这些Caspase基因相互间mRNA表达水平存在显著的的正相关关系.结论:未发现Caspase凋亡通路上相关基因mRNA表达水平在肿瘤形成过程中发生显著变化;Caspase通路上基因相互之间表达水平呈显著相关.
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| 关 键 词： | 结直肠肿瘤 结肠息内 肠息肉 半胱氨酸天冬氨酸蛋白酶/遗传学 基因表达 逆转录聚合酶链反应 |
Expression of caspase apoptosis pathway genes mRNA in colorectal cancer and polypus |
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| PAN Yi-feng,ZHANG Ming-wu,JIN Ming-juan,ZHANG Shan-chun,CHEN Kun,LI Qi-long,MA Xin-yuan,YAO Kai-yan,YU Yun-xian.Expression of caspase apoptosis pathway genes mRNA in colorectal cancer and polypus[J].Journal of Zhejiang University(Medical Sciences),2011,40(3). |
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| Authors: | PAN Yi-feng ZHANG Ming-wu JIN Ming-juan ZHANG Shan-chun CHEN Kun LI Qi-long MA Xin-yuan YAO Kai-yan YU Yun-xian |
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| Institution: | PAN Yi-feng1,ZHANG Ming-wu1,JIN Ming-juan1,ZHANG Shan-chun1,CHEN Kun1,LI Qi-long2,MA Xin-yuan2,YAO Kai-yan2,YU Yun-xian1(1.Department of Epidemiology and Health Statistics,Zhejiang University School of Public Health,Hangzhou 310058,China,2.Cancer Institute of Jiashan County,Jiashan 314100,China) |
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| Abstract: | Objective:To investigate mRNA expression of caspase apoptosis pathway genes in colorectal cancer,polypus and normal mucosa.Methods:Nineteen patients with colorectal cancer,86 patients with polypus and 10 normal controls were enrolled from 2008 to 2010.Fluorescence quantitative RT-PCR was performed to detect the mRNA expression of caspase apoptosis pathway genes(caspase-2,-3,-6,-7,-8,-9 and-10) in colorectal cancer,polypus and normal mucosa.Results:There were no statistically significant differences of demog... |
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