儿童攻击行为与COMT Val158Met及5-HTTLPR基因多态性关联分析

目的：探讨儿茶酚胺氧位甲基转移酶(COMT) 第158位密码子从缬氨酸到蛋氨酸的错义突变(Val158Met)多态性及5-羟色胺转运体（5-HTT）基因启动子区缺失/插入多态性（5-HTTLPR）是否为家庭暴力环境下儿童攻击行为的易感因素。方法：以家庭暴力环境下68例儿童作为研究对象，依据Achenbach儿童行为量表（父母卷）评分，其中24例为攻击行为高分组，44例为低分组。采用聚合酶链式反应 限制性片段长度多态性（PCR-RFLP）技术，检测两组COMT Val158Met和5-HTTLPR多态性，对所得基因型和等位基因频率进行对照和关联分析。结果：攻击行为高分组与低分组COMT Val158Met多态性的各基因型差异(χ2＝1.612，P=0.447)和等位基因频率差异(χ2=1.648，P=0.119)均无统计学意义。两组5-HTTLPR多态性的各基因型差异(χ2=1.807，P=0.405)和等位基因频率差异(χ2=0.403，P=0.527)亦无统计学意义。结论：本研究结果不支持COMT Val158Met和5-HTTLPR基因多态性是儿童攻击行为产生的易感因素。提示儿童攻击行为可能是多因素共同作用的结果。

Association between aggressive behaviors and COMT Val158Met and 5-HTTLPR polymorphisms in children

Objective To investigate the association between aggressive behaviors and catechol-O-methyltransferase(COMT) single nucleotide polymorphism at position 158 from a valine to a methionine(Val158Met) as well as serotonin(5-HT) transporter gene linked polymorphic region(5-HTTLPR) in children.Methods A total of 68 children who were exposed to domestic violence were recruited.The frequencies of genotypes and alleles of COMT Val158Met and 5-HTTLPR were examined by polymerase chain reaction(PCR) and restriction fra...