乳腺癌患者肿瘤转移相关基因1和基质金属蛋白酶9的表达及意义

目的 研究乳腺癌组织中肿瘤转移相关基因1(MTA1)和基质金属蛋白酶9(MMP-9)的表达及其与肿瘤侵袭转移之间的关系.方法 运用荧光实时定量PCR技术检测56对乳腺癌组织和配对的正常乳腺组织中MTA1、MMP-9 mRNA的表达.结果 83.9%(47/56)的乳腺癌原发灶标本MTA1基因的表达高于其配对的正常乳腺组织.85.7%(48/56)的乳腺癌原发灶MMP-9基因的表达高于配对正常乳腺组织.MTA1、MMP-9基因表达与乳腺癌的浸润、转移呈正相关(P<0.05).MTA1与MMP-9基因表达呈正相关(P<0.05).结论 联合检测MTA1、MMP-9基因表达水平,可以预测乳腺癌的浸润转移,为临床治疗和判断预后提供依据.

Correlation between expression of metastasis-associated gene 1 and matrix metalloproteinase 9 and invasion and metastasis of breast cancer

Objective To investigate the expression of metastasis-associated gene 1 (MTA1) and matrix metalloproteinase 9 (MMP-9) gene in breast carcinoma and the relationship between the expression of these genes and the invasion of breast cancer. Methods Fluorescence quantitative PCR technique was used to detect the mRNA expression of MTA1 and MMP-9 gene among 56 human breast cancer samples. Results The mRNA expression rate of MTA1 was higher in 83.9% ( 47/56 ) of the primary breast cancer tissues compared with the matched normal breast tissues. The mRNA expression of MMP-9 was higher in 85.7% (48/56) of the primary breast cancer tissues compared to the matched normal breast tissues. The over-expression of MTA1 and that of the MMP-9 gene were significantly related with the degree of differentiation,clinical stage, and lymph node metastasis of breast cancer ( all P 0. 05 ). The positive ratio of MTA1 gene is tightly associated with that of MMP-9 gene (P 0. 05). Conclusion The united detection of MTA1 and MMP-9 gene expression predicts the invasion and metastasis of breast cancer and supplies evidence for clinical therapy and judgment of prognosis. MTA1 and MMP-9 will become new targets for gene therapy.