绿色荧光蛋白标记的人卵巢癌裸鼠原位移植模型的建立

目的 应用绿色荧光蛋(GFP)基因标记人卵巢癌细胞株H08910,建立新型裸鼠原位移植瘤模型.方法 将处于对数生长期的人卵巢癌细胞株H08910/GFP 2×106个细胞注射于裸鼠腋下,收集瘤块后接种于左侧卵巢包膜下,共6只.术后利用荧光体视显微镜连续观察肿瘤生长情况,4周后处死荷瘤鼠,观察肿瘤生长及转移情况.结果 种植的原位移植瘤成瘤率100%,移植后2周可观察到左侧肋脊角处绿色荧光团,并逐渐增大,4周后,可见肿瘤侵及腹膜、网膜、肠管、脾脏、肝脏、子宫、盆腔淋巴结,转移率达66.7%.结论 成功建立新型裸鼠原位人卵巢癌的动物模型.

Establishment of a nude mouse model bearing orthotopically transplanted human ovarian carcinoma expressing green fluorescent protein

OBJECTIVE: To establish a human ovarian cancer-bearing mouse model via orthotopic transplantation of human HO-8910 cells expressing green fluorescent protein (GFP). METHODS: GFP-expressing human ovarian carcinoma HO8910/GFP cells (2 x 10(6)) in exponential phase of growth were inoculated subcutaneously in nude mice, and the generated tumor tissues were collected and transplanted below the capsule of the left ovary of 6 nude mice. The growth of the tumors was observed in vivo using a fluorescence stereomicroscope. The nude mice were sacrificed 4 weeks after transplantation to assess the tumor growth and metastasis. RESULTS: The tumors showed progressive growth at the orthotopic sites in all animals. Two weeks after the transplantation, green fluorescent mass was observed at the left costovertebral angle, and the mass increased thereafter and invaded or metastasized to the peritoneum, omentum, spleen, liver, uterus, and the pelvic lymph nodes, with a metastatic rate as much as 66.7%. CONCLUSION: The nude mouse model bearing orthotopic human ovarian carcinoma expressing GFP has been successfully established.