| 脑胶质瘤基因治疗中丙氧鸟苷对细胞系的毒性分析 |
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| 引用本文： | 索新,周劲松,何成彦,郭永川. 脑胶质瘤基因治疗中丙氧鸟苷对细胞系的毒性分析[J]. 吉林大学学报(医学版), 2003, 29(4): 442-444 |
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| 作者姓名： | 索新 周劲松 何成彦 郭永川 |
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| 作者单位： | 吉林大学中日联谊医院神经外科,吉林,长春,130021;吉林大学中日联谊医院中心研究室 |
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| 基金项目： | 吉林省科技厅科研项目;953557-5; |
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| 摘 要： | 目的 :研究丙氧鸟苷 ( GCV)对 C6BPBAG脑胶质瘤细胞、psi2细胞、psi2 STK细胞的毒性。方法 :将 C6BPBAG脑胶质瘤细胞、psi2细胞、psi2 STK细胞分别在不同浓度 GCV的培养基中培养 ,观察以上 3类细胞对 GCV的毒性反应。结果 :在有效浓度 ( 0 .0 1～ 1 0 μmol·L-1 )中 ,GCV对C6BPBAG脑胶质瘤细胞、psi2细胞几乎无伤害 ,而经改造后的 psi2 STK细胞对 GCV毒性明显提高。结论 :通过体外实验证实由逆转录病毒载体 ( STK)传导的 HSV- TK基因 ,在培养的 C6胶质瘤细胞中 ,可提高 GCV对 psi2 STK细胞的敏感性
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| 关 键 词： | 神经胶质瘤/治疗 阿昔洛韦/治疗应用 基因疗法 单纯病毒属 胸苷激酶/遗传学 |
| 文章编号： | 1671-587X(2003)04-0442-03 |
| 修稿时间： | 2002-09-19 |
Toxic Effects of GCV on Cell Lines in Gene Therapy of Brain Glioma |
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| SUO Xin ,ZHOU Jin song ,HE Cheng yan ,GUO Yong chuan. Toxic Effects of GCV on Cell Lines in Gene Therapy of Brain Glioma[J]. Journal of Jilin University: Med Ed, 2003, 29(4): 442-444 |
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| Authors: | SUO Xin ZHOU Jin song HE Cheng yan GUO Yong chuan |
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| Affiliation: | SUO Xin 1,ZHOU Jin song 2,HE Cheng yan 2,GUO Yong chuan 1 |
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| Abstract: | Objective: To study the toxic effects of Ganciclovir(GCV) on C 6BPBAG.psi2 and psi2STK. Methods: In order to observe the toxic effects of GCV on C 6BPBAG,Psi2 and Psi2STK, these cells were planted at a density of 10 4 cells per cm 2 with different concentration of GCV. Results:When the density of GCV was 0.01~10 μmol·L -1 ,GCV was almost not harmful to C 6BPBAG and psi2. However when psi2STK was transformed, the toxic effects of GCV on Psi2STK were increased singnificantly. Conclusion: According experiment in vitro , it is proved that when HSV TK gene is transferred by STK, the sensitivity of GCV to Psi2STK is improved. |
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| Keywords: | Glioma/therapy Acyclovir/therapeutic use Gene therapy Simplexvirus Thymidine kinase/genetics |
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