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| Effect of N—desulfated heparin on hepatic／renal ischemia reperfusion injury in rats | |
| 引用本文： | Zhou T,Chen JL,Song W,Wang F,Zhang MJ,Ni PH,Geng JG. Effect of N—desulfated heparin on hepatic／renal ischemia reperfusion injury in rats[J]. World journal of gastroenterology : WJG, 2002, 8(5): 897-900. DOI: 10.3748/wjg.v8.i5.897 |
| 作者姓名： | Zhou T Chen JL Song W Wang F Zhang MJ Ni PH Geng JG |
| 作者单位： | Tong Zhou,WEI Song,Feng WANG(Department of Nephrology,Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China);Jin-Lian Chen(Department of Gastroenterology,ShanghaiSixth people's Hospital, Shanghai 200233, China);Ming-Jun Zhang(Animal Laboratory, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China);Pei-Hua Ni(Department of Biochemistry, Shanghai Second Medical University, Shanghai 200025);Jian-Guo Geng(Institute of Biochemistry and Cell Biology, Shanghai 200030, China) |
| 基金项目： | 国家自然科学基金,卫生部科研项目 |
| 摘 要： | AIM：To invesigate the effect of N-desulfated heparin on hepatic/renal ischemia and reperfusion injury in rats.METHODS:Using rat models of 60minutes hepatic of renal ischemia followed by 1h,3h,6hand24h reperfusion.animals were randomly divided into following groups,the sham operated controls,ischemic group receiving only normal saline,and treated group receiving N-desulfated heparin at a dose of 12mg/kg at 5minutes before reperfusion.P-selectin expression was detected in hepatic/renal tissues with immunohistochemistry method.RESULTS:P-selectin expression,serumALT,AST,BUNand Cr levels were significantly increased during60minute ischemia and 1h,3h,6hand24hreperfusion.while the increment was significantly inhibited,and hepatic/renal pathology observed by light microscopy was remarkably improved by treatment with the N-desulfated heparin.Furthermore.the heparin was found no effects on PT and KPTT.CONCLUSION:P-selectin might mediate neutrophil infitration and contribute to hepatic/renai ischemia and reperfusion.THe N-desulfated heparin might prevent hepatic/renal admage induced by ischemia and reperfusion injury without significant anticoagulant activity. |
| 关 键 词： | N－硫肝磷酯 肝缺血 肾缺血 再灌注损伤 治疗 动物实验 |
| 收稿时间： | 2002-03-25 |
Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats |
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| Zhou Tong,Chen Jin-Lian,Song Wei,Wang Feng,Zhang Ming-Jun,Ni Pei-Hua,Geng Jian-Guo. Effect of N-desulfated heparin on hepatic/renal ischemia reperfusion injury in rats[J]. World journal of gastroenterology : WJG, 2002, 8(5): 897-900. DOI: 10.3748/wjg.v8.i5.897 | |
| Authors: | Zhou Tong Chen Jin-Lian Song Wei Wang Feng Zhang Ming-Jun Ni Pei-Hua Geng Jian-Guo |
| Affiliation: | 1. Department of Nephrology,Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China 2. Department of Gastroenterology,ShanghaiSixth people's Hospital, Shanghai 200233, China 3. Animal Laboratory, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025, China 4. Department of Biochemistry, Shanghai Second Medical University, Shanghai 200025 5. Institute of Biochemistry and Cell Biology, Shanghai 200030, China |
| Abstract: | AIM: To investigate the effect of N-desulfated heparin on hepatic/renal ischemia and reperfusion injury in rats. METHODS: Using rat models of 60 minutes hepatic or renal ischemia followed by 1 h,3 h,6 h and 24 h reperfusion,animals were randomly divided into following groups,the sham operated controls,ischemic group receiving only normal saline,and treated group receiving N-desulfated heparin at a dose of 12 mg/kg at 5 minutes before reperfusion. P-selectin expression was detected in hepatic/renal tissues with immunohistochemistry method. RESULTS: P-selectin expression, serum ALT, AST, BUN and Cr levels were significantly increased during 60 minute ischemia and 1 h, 3 h, 6 h and 24 h reperfusion,while the increment was significantly inhibited,and hepatic/renal pathology observed by light microscopy was remarkably improved by treatment with the N-desulfated heparin. Furthermore,the heparin was found no effects on PT and KPTT. CONCLUSION: P-selectin might mediate neutrophil infiltration and contribute to hepatic/renal ischemia and reperfusion. The N-desulfated heparin might prevent hepatic/renal damage induced by ischemia and reperfusion injury without significant anticoagulant activity. |
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