吡格列酮对高糖高胰岛素诱导的肥大心肌细胞中CT-1的影响

目的：观察心肌营养素 1(CT 1)在高糖高胰岛素诱导的肥大心肌细胞中的表达，以及吡格列酮对其表达的影响。方法：体外培养乳鼠心肌细胞，分组给药后，检测心肌细胞肥大的指标：心肌细胞的表面积(图像分析法)、蛋白含量(考马斯亮蓝法)、心房利钠因子(ANF) mRNA的表达(RT PCR法)；同时用半定量RT PCR检测CT 1 mRNA的表达。结果：高糖高胰岛素组心肌细胞表面积、蛋白含量、ANF mRNA表达以及CT 1 mRNA水平均升高(P<0.01)，而吡格列酮治疗组降低(P<0.05)。结论：高糖高胰岛素诱导的肥大心肌中CT 1表达上升，而吡格列酮能抑制其表达，推测CT 1可能参与了高糖高胰岛素所引起的心肌肥大的发生，而吡格列酮抑制心肌肥大的作用可能是通过抑制CT 1介导的。

Effects of pioglitazone on cardiotrophin-1 expression in hypertrophic cardiomyocytes induced by high glucose and insulin

Objective: To study the expression of cardiotrophin-1(CT-1) in hypertrophic cardiac myocytes induced by high glucose and insulin,and the effect of pioglitazone on the expression of cardiotrophin-1(CT-1).Methods: The cardiac myocytes were cultured in vitro as a model.Cellular surface,protein content and atrial natriuretic factor mRNA were measured as the cellular hypertrophic parameters.Meanwhile,the expression of cardiotrophin-1 mRNA was assayed with semiquantitative RT-PCR.Results: After treatment with high glucose and insulin,cellular surface,protein content and expression of atrial natriuretic factor mRNA increased significantly(P<0.01).Pioglitazone inhibited all these above effects effectively(P<0.05).Conclusion: The results suggest that cardiotrophi-1 is involved in the development of myocardial hypertrophy induced by high glucose and insulin.And pioglitazonemay inhibit myocardial hypertrophy by restraining the expression of cardiotrophin-1.