Measurement of IgM and IgG antibodies to Yersinia by solid-phase radioimmunoassay

Solid-phase radioimmunoassays for IgM and IgG antibodies to Yersinia enterocolitica serotype 3. Y. enterocolitica serotype 9, and Yersinia pseudotuberculosis IA are described. Bacteria were adsorbed onto polystyrene balls, and serum antibodies bound to the antigen-coated balls were quantitated by their capacity to bind 125I-labeled antibodies to human immunoglobulins.