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| 银染RT-PCR检测胃癌淋巴结微转移方法的探讨 | |
| 引用本文： | 陈瑞川,林岚,苏金华. 银染RT-PCR检测胃癌淋巴结微转移方法的探讨[J]. 肿瘤, 2000, 20(3): 206-208 |
| 作者姓名： | 陈瑞川 林岚 苏金华 |
| 作者单位： | [1]厦门大学生命科学学院抗癌中心 [2]厦门大学生命科学学院生物系 |
| 基金项目： | 福建省卫生厅基金!(96075)，厦门大学肿瘤细胞工程开放实验室基金资助项目 |
| 摘 要： | 目的 建立ＲＴ－ＰＣＲ检测胃周淋巴结胃癌转移细胞的方法，评价其临床应用价值。方法 以ＭＵＣ１ ｃＤＮＡ的引物建立了ＲＴ－ＰＣＲ扩增体系及ＰＡＧＥ－银染检测ＰＣＲ扩增产物的方法，并作了优化；在对扩增体系的检测特异性和敏感性作了分析后，初步检测了临床样品。结果 该扩增体系具有较好的特异性，初步检测了临床样品。结果 该扩增体系具有较好的异性，敏感性可达１０＾－６μｇＲＮＡ，相当于从１０＾５个淋巴细胞中检 |
| 关 键 词： | 胃肿瘤 淋巴结微转移 银染RT－PCR |
DETECTINGGASTRIC CANCER MICROMETASTASES IN AXILLARY LYMPH NODES BY RT-PCR AMPLIFICATION AND SILVERSTAINING |
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| CHEN Ruichuan,LIN Lan,SU Jinhua,et al.. DETECTINGGASTRIC CANCER MICROMETASTASES IN AXILLARY LYMPH NODES BY RT-PCR AMPLIFICATION AND SILVERSTAINING[J]. Tumor, 2000, 20(3): 206-208 | |
| Authors: | CHEN Ruichuan LIN Lan SU Jinhua et al. |
| Affiliation: | CHEN Ruichuan1,LIN Lan2,SU Jinhua1,et al. 1 Cancer Res.Center,2 Dept.of Biology,Xiamen Univ Xiamen 361005,China |
| Abstract: | Objective To develop a RTPCR method for detecting gastric cancer micrometastases in axillary lymph nodes.Methods The RTPCR was set up for amplifying MUC1 mRNA and the PAGEsilver staining for detecting amplification products.After optmizing the PCR system,the specificity and sensitivity were investigated by amplifying different selected samples and serial dilution method respectively.This method was performed on axillary lymph nodes and compared the results with that of pathological examination for estimating the reliability.Results This system showed a good specificity and a high sensitivity of detecting one gastric cancer cell out of 105 lymphocytes.The results corresponded with the pathological examination suggesting a good reliability of this method.Conclusion The RT PCR method possesses a high reliability and promise in clinical appilcation. |
| Keywords: | Gastric neoplasms Lmyph node RT-PCR Gene MUC1 Neoplasm metastasis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |