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| Smo基因在小鼠胚胎颌面部的表达 | |
| 引用本文： | 杜娟,刘淑红,范文红,沈岩,范志朋,范明,王松灵. Smo基因在小鼠胚胎颌面部的表达[J]. 口腔颌面外科杂志, 2005, 15(1): 13-15,20 |
| 作者姓名： | 杜娟 刘淑红 范文红 沈岩 范志朋 范明 王松灵 |
| 作者单位： | 首都医科大学口腔医学院基因治疗分子生物学实验室,北京,100050;军事医学科学院基础所脑保护与可塑性研究实验室,北京,100850;国家人类基因组北方研究中心,北京,100176;首都医科大学口腔医学院基因治疗分子生物学实验室,北京,100050;国家人类基因组北方研究中心,北京,100176 |
| 基金项目： | 863重大专项子课题资助(2002BA711A07-11) |
| 摘 要： | 目的:探讨Smoothened(Smo)基因在小鼠胚胎颌面部正常发育中的表达。方法: 应用免疫组织化学ABC法和图像分析系统研究Smo基因在小鼠胚胎11.5、13.5及17.5d颌面部的表达情况。结果:Smo基因在胚胎11.5、13.5及17.5d的颌面上下颌突均有表达,且较对照组有显著性差异(P<0.05),但在上下颌突之间表达没有显著性差异,在上皮和结缔组织之间表达基本无差异。结论: Smo基因在小鼠胚胎颌面部正常发育有明显表达,提示Smo基因可能参与颌面部生长发育。 |
| 关 键 词： | Smoothened 小鼠胚胎 颌面部 免疫组化 图像分析 |
| 文章编号： | 1005-4979(2005)01-0013-03 |
Expression of Smoothened in the Maxillofacial Region of Mouse Embryo |
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| DU Juan,Liu Shu-hong,FAN Wen-hong,SHEN Yan,FAN Zhi-peng,FAN Ming,WANG Song-ling. Expression of Smoothened in the Maxillofacial Region of Mouse Embryo[J]. Chinese Journal of Oral and Maxillofacial Surgery, 2005, 15(1): 13-15,20 | |
| Authors: | DU Juan Liu Shu-hong FAN Wen-hong SHEN Yan FAN Zhi-peng FAN Ming WANG Song-ling |
| Affiliation: | DU Juan1,LIU Shu-hong2,FAN Wen-hong2,SHEN Yan3,FAN Zhi-peng1,3,FAN Ming2,WANG Song-ling1 |
| Abstract: | Objective: To observe the expression of Smoothened(Smo) in the maxillofacial region of mouse embryo. Methods: ABC immunocytohistochemistry method and image analysis were used to detect the expression of Smo in maxillofacial region from 11.5th,13.5th to17.5th day of mouse embryo stage. Results: The positive expression was found in maxillary and mandibular region in all three stages (P<0.05). No significant difference of expression between maxilla and mandible of mouse embryo was found. And there was no distinct difference of expression between epithelial and connective tissue. Conclusion: Smo was positive expression in maxillofacial region which was considered to involve in development of this region. |
| Keywords: | Smoothened mouse embryo maxillofacial region immunohistochemistry image analysis |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |