复肾颗粒对肾间质纤维化大鼠肾脏TRAF6表达的影响

目的:观察复肾颗粒对单侧输尿管结扎致肾间质纤维化模型大鼠的肾脏保护作用,探讨其机制。方法:将60只雄性Wi st ar大鼠行单侧输尿管结扎术,分为模型组、假手术组、贝那普利组[10 mg/(kg·d)]、复肾颗粒1组[7.5 g/(kg·d)]、复肾颗粒2组[15 g/(kg·d)]、复肾颗粒3组[30 g/(kg·d)]。各组于灌胃给药及灌胃生理盐水14天后处死大鼠,收集大鼠血清进行血肌酐(Scr)、尿素氯(BUN)检测;取结扎侧肾脏,进行HE和Masson染色,观察肾小管间质纤维化程度,RT-PCR和West er n bl ot法检测肾组织TRAF6和α-SMA的表达。结果:与假手术组相比,模型组大鼠血清中Scr和BUN含量均增加(P0.01),肾小管损伤程度较重(P0.05),肾组织TRAF6和α-SMA表达明显增多(P0.05);与模型组比较,不同剂量复肾颗粒作用后大鼠血清中Scr和BUN含量均下降(P0.05),肾小管损伤程度有所缓解(P0.05),肾组织内TRAF6和α-SMA表达降低(P0.05),随着药物剂量的增加,各指标程度加重。结论:复肾颗粒可明显减轻肾间质炎症细胞浸润及间质纤维化,延缓肾功能损害,复肾颗粒对单侧输尿管结扎大鼠肾小管间质纤维化有保护作用,其机制可能与抑制TRAF6和α-SMA表达有关。

The Effects of FuShen Granules on the Expression of TRAF6 in the Kidney of the Rats with Renal Interstitial Fibrosis

Objective:To observe the protective effects of FuShen granules on the kidney of rat model with renal interstitial fibrosis induced by unilateral ureteral ligation, and to explore its mechanism. Methods:Sixty male Wistar rats accepted unilateral ureteral ligation, they were divided into the model group, sham operation group, benazepril group [10 mg/(kg·d)], FuShen granules group No.1 [7.5 g/(kg·d)], FuShen granules group No.2 [15 g/(kg·d)], FuShen granules group No.3 [30 g/(kg·d)]. The rats in different groups were killed in 14 days after they were lavaged of medicine and physiological saline, creatinine (Scr) and urea nitrogen (BUN) of the rate were measured after collecting the serum of the rats, the degree of renal interstitial fibrosis were observed after HE and Masson staining of the ligated kidney, the expressions of TRAF6 and α -SMA in renal tissues were detected by RT-PCR and Western blot. Results:Compared with the sham operation group, the contents of serum Scr and BUN in the model group increased significantly (P<0.01), renal tubular damage was more severe (P<0.05), and the expressions of TRAF6 and α -SMA in renal tissue significantly increased (P<0.05). Compared with the model group, the contents of Scr and BUN in the serum of the rats decreased after treated with different doses of FuShen granules (P<0.05), renal tubular damage was alleviated (P<0.05), the expressions of TRAF6 andα-SMA in renal tissue decreased (P<0.05). With the increase of drug dosage, the indexes increased. Conclusion:FuShen granules could significantly relieve renal interstitial inflammatory cell infiltration and interstitial fibrosis obviously, and delay renal function damage, it could protect renal interstitial fibrosis of the rats induced by unilateral ureteral ligation, and the mechanism might be related to inhibiting the expressions of TRAF6 andα-SMA.