| 豚鼠胆色素结石模型的肠道免疫屏障研究 |
| |
| 引用本文： | 于浩,俊金哲,吴硕东. 豚鼠胆色素结石模型的肠道免疫屏障研究[J]. 中国医科大学学报, 2010, 39(3): 187-190 |
| |
| 作者姓名： | 于浩 俊金哲 吴硕东 |
| |
| 作者单位： | 1. 中国医科大学,附属盛京医院第一微创胆道外科,沈阳,110004
2. 中国医科大学,附属第四医院肝胆胰外科,沈阳,110032 |
| |
| 摘 要： | 目的 探讨肠黏膜免疫屏障与胆色素结石形成间的相关性及其可能机制.方法 豚鼠80只随机分为对照组(CON)、致石组(PS)、肠黏膜保护组(GLN),分别给予正常饲料、胆色素结石致石饮食、添加肠黏膜保护剂谷氨酰胺的致石饮食,饲养8周建立胆囊胆色素结石模型.检测并比较各组胆结石的成石率,绒毛形态,高倍镜下(x400)肠黏膜固有层和上皮内的、CD3+T细胞数、CD40+B细胞数和IgA阳性浆细胞数.结果 致石组成石率为68.4%,明显高于对照组20.0%和保护组36.8%(P＜0.05).相比对照组,成石组肠壁各层变薄,炎性表现.阳性T淋巴细胞由每高倍视野(21.8±2.5)个降至(11.1±3.4)个,P＜0.01;B淋巴细胞由每高倍视野(12.9±2.0)个降至(10.7±3.6)个,P＜0.01;浆细胞数由每高倍视野(12.4±3.4)个降至(10.7±3.5)个,P＜0.01.肠黏膜保护组肠壁炎性表现轻于致石组,各指标均较致石组高(P＜0.01),有显著性差异.结论 结石形成过程中,存在肠黏膜机械屏障和免疫屏障功能异常.谷氨酰胺可改善豚鼠的肠黏膜屏障功能,降低胆色素结石的形成率.
|
| 关 键 词： | 肠黏膜免疫屏障 肠黏膜机械屏障 胆色素结石 |
Intestinal Mucosal Immune Barrier in Guinea Pig Model of Pigment Gallstone |
| |
| YU Hao , JUN Jin-zhe , WU Shuo-dong. Intestinal Mucosal Immune Barrier in Guinea Pig Model of Pigment Gallstone[J]. Journal of China Medical University, 2010, 39(3): 187-190 |
| |
| Authors: | YU Hao JUN Jin-zhe WU Shuo-dong |
| |
| Affiliation: | YU Hao1,JUN Jin-zhe2,WU Shuo-dong1 (1. First Department of Minimally Invasive Biliary Surgery,Shengjing Hospital,China Medical University,Shenyang 110004,China,2. Department of Hepatobiliary Pancreatic Surgery,The Fourth Affiliated Hospital,Shenyang 110032,China) |
| |
| Abstract: | Objective To explore the correlation between intestinal mucosal immune barrier and pathogenesis of pigment gallstone and its possible mechanism. Methods Eighty guinea pigs were randomly divided into control group(group CON),pigment gallstone group(group PS),and intestinal mucosal protection group(group GLN). The guinea pigs were fed with normal diet in group CON,pigment gallstonein ducing diet in group PS,and glutamine-supplemented diet in group GLN for 8 weeks. The guinea pig model of pigment gallstone was... |
| |
| Keywords: | intestinal mucosal immune barrier intestinal mucosal mechanical barrier pigment gallstone |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
