支原体肺炎患儿血清TNF-α、IL-6、IL-8的检测及其临床意义

目的 检测支原体肺炎(MPP)患儿血清中肿瘤坏死因子-a(TNF-a)、白细胞介素-6(IL-6)、白细胞介素-8(IL-8)水平,探讨其临床意义.方法 应用ELISA双抗体夹心法检测44例MPP患儿(研究组)和36例健康儿童(对照组)血清中TNF-α、IL-6、IL-8水平.结果 研究组MPP患儿急性期血清TNF-α、IL-6、IL-8分别为(29.22±1.26)、(20.18±1.32)、(28.01±1.38)pg/ml,高于对照组的(7.98±2.30)、(6.21±0.72)、(7.82±2.00)pg/ml,差异有统计学意义(P＜0.05)；研究组恢复期血清TNF-α、IL-6、IL-8分别为(11.02±1.46)、(8.86±1.02)、(10.54±1.07)pg/ml,显著低于急性期(P＜0.05)；虽然高于对照组,差异无统计学意义；12例MPP肺纤维化患儿胸水TNF-α、IL-6、IL-8分别为(49.02±2.20)、(32.28±2.54)、(45.90±2.86)pg/ml,较无肺纤维化者的(28.80±1.92)、(26.06±1.77)、(28.86±2.75) pg/ml,显著增高(P＜0.05),12例MPP肺纤维化血清中TNF-α、IL-6、IL-8分别为(30.18±2.86)、(27.84±2.86)、(28.42±3.20)pg/ml,低于其胸水(P＜0.05)；6例存在胸腔积液但无肺纤维化改变的患儿,胸水中TNF-α、IL-6、IL-8水平与血清中差异无统计学意义.结论 TNF-α、IL-6、IL-8在MPP的发生、发展中可能起重要作用,并且与肺部有无纤维化形成有关,故在诊断、治疗、预后方面有重要意义.

Detection of TNF-a,IL-6,IL-8 levels in serum of children with Mycoplasma pneumoniae and its clinical significance

OBJECTIVE To investigate the levels of tumor necrosis factor-a(TNF-a),interleukin-6(IL-6),interleukikn-8(IL-8) in serum of children with Mycoplasma pneumoniae pneumonia(MPP). METHODS The levels of TNF-a,IL-6 and IL-8 in serum were measured by means of double-antibody sandwich enzyme-linked immunosobent assay(ELISA) in 44 children with MPP and in 36 healthy children who were taken in the control group. RESULTS The levels of TNF-a,IL-6 and IL-8 in all samples with MPP at acute stage were(29.22±1.26),(20.18±1.32) and(28.01±1.38)pg/ml,respectively,which were significantly higher than(7.98±2.30),(6.21±0.72) and(7.82±2.00) pg/ml in control group,respectively(P<0.05),but were significantly lower than(11.02±1.46),(8.86±1.02) and(10.54±1.07) pg/ml at recovery phase,respectively(P<0.05).And there were no significant difference between MPP group at recovery phase and control group(P>0.05).In 12 MPP patients with lung fibrosis,the levels of TNF-a,IL-6,IL-8 pleural effusion at(49.02±2.20),(32.28±2.54) and(45.90±2.86)pg/ml,respectively,was higher than those of patients with no fibrosis at(28.80±1.92),(26.06±1.77) and(28.86±2.75) pg/ml,respectively(P<0.05),and also higher than the levels in serum of MPP patients at(30.18±2.86),(27.84±2.86),(28.42±3.20)pg/ml(P<0.05).While for 6 patitens with pleural effusion but no lung fibrosis,there was no statistically significant difference in the levels of TNF-a,IL-6 and IL-8 between serum and pleural effusion(P>0.05). CONCLUSION TNF-a,IL-6 and IL-8 may play an important role in the pathogenesis of MPP and may contribute largely to the development of the lung fibrosis.Thus,the levels of TNF-a,IL-6 and IL-8 may have great significance in the diagnosis,treatment and prognosis of the MPP.