实时荧光定量PCR检测EV71肠道病毒在不同类型样本中检测率的分析

目的 探讨肠道病毒71型(EV 71)检测方法和样本收集对检测结果的影响.方法 采集疑拟患者鼻咽拭子和大便样本,应用实时荧光定量PCR方法检测.结果 结果EV71型肠道病毒大便检出率为46.2％,鼻咽拭子为5.61％,大便的检出率明显高于鼻咽拭子;差异有统计学意义(x2=61.51＞x20.01.1,P＜0.01).结论 对HFMD的诊断建议首选采集粪便标本,应用实时荧光定量PCR方法进行检测,可减少漏诊率,提高灵敏度.

Detection rate of enterovirus 71 in different types of samples by real-time fluorescence quantitative PCR

OBJECTIVE To investigate the effects of different detection methods and samples on the results of enterovirus 71(EV71) detection.METHODS The stool and the nasopharyngeal swab samples were detected by real-time fluorescence quantitative polymerase chain reaction(RT·PCR).RESULTS The positive rate of hand-foot-mouth disease(HFMD) caused by EV71 was 46.2% in the stools,and 5.61% in the nasopharyngeal swab.The determined positive rate in the stools was higher that in the nasopharyngeal swab,the difference statistically significant(χ2=61.51>χ20.01,1,P<0.01).CONCLUSION To diagnose HFMD,it is recommended that excrement specimen should be preferred for EV71 detection by real-time fluorescence quantitative PCR,which may reduce the rate of missed diagnosis and raise the detection sensitivity.