胰岛素对滋养层细胞凋亡的调节作用及机制

目的 :探讨胰岛素对培养的滋养细胞凋亡的影响及可能的机制。方法 :将培养的妊娠早期滋养层细胞 ,分为正常对照组(细胞 +培养液 )、H2 O2 组 (细胞 +培养液 +H2 O2 )和胰岛素 +H2 O2 组 (细胞 +H2 O2 +胰岛素 )。采用透射电镜观察及流式细胞术 ,观察H2 O2 诱导的细胞凋亡及胰岛素对H2 O2 诱导的细胞凋亡的抑制作用。并检测胰岛素对滋养细胞caspase 3的活性及Bcl 2蛋白表达的影响。结果 :H2 O2 可诱导培养的滋养细胞凋亡 ,透射电镜下可见特征性的细胞核改变。胰岛素可显著抑制H2 O2 诱导的细胞凋亡 ,流式细胞仪检测其凋亡率较H2 O2 组显著下降 (P <0 .0 1)。H2 O2 组滋养细胞中caspase 3的活性较对照组显著增高 (P <0 .0 1) ,而Bcl 2蛋白的表达则较对照组显著下降 (P <0 .0 1)。结论 :胰岛素可明显抑制H2 O2诱导的滋养细胞凋亡 ,其机制可能与降低caspase 3的活性和促进Bcl 2蛋白的表达有关

Effect of insulin on apoptosis of cultured human trophoblast cells and its mechanism

AIM: To explore the effect of insulin on apoptosis of cultured human trophoblast cells and its possible mechanism. METHODS: Human trophoblast cells from early pregnancy women were cultured and divided into 3 groups; normal control group; H 2O 2 treatment group and insulin plus H 2O 2 treatment group. H 2O 2 was used to induce apoptosis of trophoblasts cells. Apoptotic rate was detected by flow cytometry. The effects of insulin on Bcl 2 expression and caspase 3 activity were also detected. RESULTS: H 2O 2 might induce apoptosis of trophoblast cells and typical morphological features of apoptotic cells was observed wnder electron microscope. Flow cytometry detection exhibited that insulin could reduce markedly H 2O 2 induced apoptotic rate of trophoblasts cells( P <0.01). Bcl 2 expression rate in H 2O 2 treatment group was significanty lower than that in control group( P <0.01), while caspase 3 activity was distinctly higher than that in control group( P <0.01). CONCLUSION: Insulin could inhibit apoptosis of human tropoblasts cells induced by H 2O 2, which be may through decreased caspase 3 activity and increased Bcl 2 protein expression.