氯喹对烟雾吸入伤大鼠肺细胞膜ATP酶活性和丙二醛含量的影响

目的：探讨氯喹对烟雾吸入伤大鼠肺细胞膜ＡＴＰ酶活性及丙二醛含量的影响，方法：８０只Ｗｉｓｔａｒ大鼠随机分成正常对照组，烟雾吸入伤１，３，６，１２和２４ｈ组以及氯喹治疗６ｈ和１２ｈ组，分别于各时相点活杀动物，取肺制备膜制剂，用生化比色法测定膜上Ｎａ＾＋，Ｋ＾＋－ＡＴＰ酶Ｍｇ＾２＋－ＡＴＰ酶和Ｃａ＾２＋－ＡＴＰ酶活性，用比色法测定膜上丙醛含量，并用定磷法测定膜总磷脂含量，结果：烟雾吸入伤后，肺细胞膜３

Influences of chloroquine on the activities of adenosine triphosphatases and the content of malondiadehyde in pulmonary cellular membrane of rats with smoke inhalation injury

OBJECTIVE:To explore the influences of chloroquine on the activities of adenosinetriphosphatases(ATPases) and the content of malondialdehyde(MDA) on rats model of smoke inhalation injury.METHODS:Eighty wistar rats were randomly divided into normal control group,injurious groups at 1,3,6,12 and 24h following smoke inhalation,and chloroquine treatment groups(chloroquine was purchased from Sigma,10mg/kg,immediatly injected intraperitoneally after smoke inhalation) at 6h and 12h following smoke inhalation.The rats were killed at each time site and the lungs were incised for preparing pulmonary cellular membrane,the activities of Na ,K -ATPase,Mg 2 -ATPase and Ca 2 -ATPase were detected by biochemistry-chromatometric method,the content of MDA was assayed by chromatometric method and the content of total phosphatide of pulmonary cellular membrane was measured by quantified phosphorus.RESULTS:The activities of three kinds of ATPases were declined significantly from 1 hour to 24 hours after smoke inhalation injury(P<0.01),with increasing in the content of MDA and decreasing in the content of total phosphatide of pulmonary cellular membrane,but the activities of Na ,K -ATPase and Ca 2 -ATPase and the content of total phosphatide were increased and the content of MDA was decreased in 6h group of chloroquine treatment while comparing with 6h group of smoke inhalation injury(P<0.05).CONCLUSION:The decomposition of phosphatide,the increase in the content of MDA and the decreases in the activities of ATPases in pulmonary cellular membrane might be important factors of the occurrence and the development of smoke inhalation injury.Chloroquine might prevent and treat smoke inhalation injury for partially improving the activities of ATPases,decreasing the content of MDA of pulmonary cellular membrane and stabilizing pulmonary cellular membrane.