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Association of ALOX5 gene polymorphisms in promoter with susceptibility to asthma and effect of LTRA in children

??Objective??To study the relationship of -1708 SNP and the repeat sequence polymorphism of Sp1/Egr1 binding site of -176/-147 in the promoter region of ALOX5 with the susceptibility to asthma??clinical characteristics and LTRA effects. Methods??A total of 243 children with asthma in the General Hospital of Shenyang Military Command from October 2016 to January 2018 were selected as case group and 228 non-asthmatic children as control group. Polymerase chain reactions??PCRs?? and sequencing methods were used to classify the ALOX5 -1708 and Sp1/Egr1 binding sites. The clinical characteristics were collected??and the anti-asthma treatments were given?? the gene polymorphisms??clinical characteristics and the effects of LTRA were analyzed. Results????1??The frequency of non 5/5 genotype of -176/-147 in case group was significantly higher than that in control group??OR??1.485?? 95%CI??1.018-2.167??χ2??4.225?? P??0.040??. ??2??The frequency of G/A+A/A and G/A in the moderate and severe groups was both significantly higher than that in the mild group??χ2??4.832?? 4.565?? P??0.028?? 0.033????the 4/6 genotype frequency in the moderate and severe group was lower than that of the 5/5 genotype??while the 5/6 genotype frequency was higher than that of the 5/5 genotype??the differences being statistically significant??χ2??3.643??3.888??P??0.056??0.049??. Conclusions??The polymorphism of ALOX5 Sp1/Egr1 binding site repeat sequence may be related to the incidence and severity of asthma??allergy??family history and LTRA effect in children. The polymorphism of ALOX5 -1708 may be related to the severity of asthma??lung function and effect of LTRA.