| HPLC-ELSD法测定杜仲皮、叶和种子中桃叶珊瑚苷的含量 |
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| 引用本文： | 刘建斌 孙文基. HPLC-ELSD法测定杜仲皮、叶和种子中桃叶珊瑚苷的含量[J]. 药物分析杂志, 2005, 25(12): 1507-1509 |
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| 作者姓名： | 刘建斌 孙文基 |
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| 作者单位： | 西北大学陕西省生物医药重点实验室,西安710069 |
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| 基金项目： | 陕西省中医管理局项目（No.16）资助. |
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| 摘 要： | Objective：To determine the content of aucubin in the barks, leaves and seeds of Eucommia ulmoides with HPLC-ELSD. Method： An HPLC-ELSD method was set up using Inertsil ODS-3 C18 （4.6 mm × 250 mm, 5μm） column. The mobile phase consisted of methanol - water（8： 92） ,with a flow rate of 1.0 mL·min^-1. The temperature of drift tube heater was 112. 9 ℃and the gas was flowing at the rate of 2. 8 L · min^-1. Results：The linear range of aucubin determination was 1.04 ～ 20. 9μg. The barks, leaves and seeds of Eucommia ulmoides were analyzed,the contents in them were 0. 999%, 1. 031% and 2.498%. The average recovery of aucubin were 98.2% ,97.3% ,97.5% ;RDS were 1.8% ,2. 1% ,2.0%. Conclusion：The method is simple and rapid with reproducibility for the determination of aucubin in the barks,leaves and seeds of Eucommia ulmoides.
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| 关 键 词： | HPLC-ELSD法 测定 杜仲 皮 叶 种子 桃叶珊瑚苷 |
| 修稿时间： | 2005-11-08 |
HPLC-ELSD Determination of Aucubin in the Barks, Leaves and Seeds of Eucommia ulmoides |
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| LIU Jian - bin, SUN Wen - ji. HPLC-ELSD Determination of Aucubin in the Barks, Leaves and Seeds of Eucommia ulmoides[J]. Chinese Journal of Pharmaceutical Analysis, 2005, 25(12): 1507-1509 |
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| Authors: | LIU Jian - bin SUN Wen - ji |
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| Abstract: | |
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| Keywords: | Eucommia ulmoides barks leaves seeds aucubin HPLC-ELSD |
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