内陆与沿海地区自然环境中战备消毒包储存时限的实验研究

目的 探讨战备消毒包在内陆和沿海地区自然环境中的储存时限及细菌的来源.方法 准备双层棉布手术敷料包、器械包、敷料器械混合包,预真空压力蒸气灭菌后分别存放在自然环境战备仓库储存箱内,分别于灭菌后不同时限点随机取3种规格消毒包各3个进行细菌培养；同时对战备仓库空气、储存箱进行取样培养,并进行细菌同源性研究.结果 灭菌消毒包储存时限内陆地区40d、沿海地区24d;两地区战备消毒包、仓库空气和储存箱优势菌属为革兰阳性杆菌和球菌；消毒包内培养出的细菌与空气、储存箱分离出来的相同菌种不同菌株之间的16～23S rRNA ITS(internal transcribed spacer,转录间隔区)序列相似性内陆地区为97％～100％、沿海地区为98％～99％.结论 不同地区战备消毒包的储存环境对其储存时限具有重要的影响,战备消毒包内细菌来源于空气,针对性储存策略是战备消毒包有效储存的重要保证.

Investigation on expiration time of sterile packages of strategic reserve between inland region and littoral under natural environment

Objective To study expiration time and the origin of bacteria for the sterile packages of strategic reserve between inland region and littoral under natural environment.Methods We prepared operation dressing package,apparatus package as well as dressing and apparatus mixed package with double- cotton and to be deposited in storage boxes of combat readiness warehouse under natural environment after pulsing vacuum steam sterilization.Three packages of every kind were put in a storage boxes and samples were taken from the packages and underwent bacterial culture at different time-points.Meanwhile,the bacteria strains in combat readiness warehouse' air and in the storage boxes were cultured and performed with homology analysis.Results Expiration time of sterile packages of strategic reserve was 40d in inland region whereas 24d in littoral.The major bacteria were G+ bacilli and G+ staphylococus in sterile packages of strategic reserve,combat readiness warehouse' air and storage boxes in two regions.In inland region,the 16～23SrRNA ITS sequences of bacteria strains in sterile packages shared about 97％～100％ homology with which between the air bacteria strains in combat readiness warehouse and the storage boxes whereas it was about 98％～99％ homology in littoral.Conclusions The storage environment is the most influential factor on expiration time of sterile packages of strategic reserve.The bacteria in sterile packages of strategic reserve came from the air of combat readiness warehouse.To ensure the expiration time of sterile package of strategic reserve,corresponding management methods are important guarantee which can provide new methods about management on strategic reserve.