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| 视网膜神经节细胞体外培养、纯化及生物学特性研究 | |
| 引用本文： | Wu Y,Ge J,Qiu P,Li Y,Lin M,Guo Y. 视网膜神经节细胞体外培养、纯化及生物学特性研究[J]. 中华眼科杂志, 1999, 35(3): 190-193,10 |
| 作者姓名： | Wu Y Ge J Qiu P Li Y Lin M Guo Y |
| 作者单位： | |
| 基金项目： | 国家自然科学基金,广东省科委重点攻关项目基金,广东省中医药管理局基金 |
| 摘 要： | 目的 建立体外视网膜神经节细胞（ｒｅｔｉｎａｌｇａｎｇｌｉｎｃｅｌｌｓ,ＲＧＣｓ）培养和纯化模型。方法 出生后１－３天Ｓｐｒａｇｕｅ－Ｄａｗｌｅｙ（ＳＤ）乳鼠,ＲＧＣｓ在ｂａｓａｌｍｅｄｉｕｍｅａｇｌｅ（ＢＭＥ）培养基中培养,相差显微镜下观察细胞生长规律。羊抗鼠单克隆抗体ＦＩＴＣ－Ｔｈｙ－１,１鉴定ＲＧＣｓ并通过Ｔｈｙ１．１单抗进行ＲＧＣｓ的分离纯化,四唑盐（ｍｏｎｏｔｅｔｒａｚｏｌｉｕｍ,ＭＴＴ |
| 关 键 词： | 视网膜 神经节细胞 RGCS 细胞培养 青光眼 |
The experimental research of purification and character of cultured retinal ganglion cell |
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| Wu Y,Ge J,Qiu P,Li Y,Lin M,Guo Y. The experimental research of purification and character of cultured retinal ganglion cell[J]. Chinese Journal of Ophthalmology, 1999, 35(3): 190-193,10 | |
| Authors: | Wu Y Ge J Qiu P Li Y Lin M Guo Y |
| Affiliation: | Zhongshan Ophthalmic Center, Sun Yat-sun University of Medical Sciences, Guangzhou 510060. |
| Abstract: | OBJECTIVE: To establish a cell line and purification model of retinal ganglion cells (RGCs) in vitro. METHOD: RGCs from Sprague Dawley neonatal rats (postnatal 1 - 3 days) were cultured in basal medium eagle (BME) basal medium. The growth regularity of RGCs in vitro was observed under phase-contrast microscope. RGCs were purified by Thy 1.1 with FITC antibody and detected under fluorescent microscope and phase-contrast microscope. RESULTS: Higher density of retinal cells and tectal extract facilitate cultured RGCs to survive. The purification rate of retinal ganglion cells in the experiment arrived at 95 percent. CONCLUSION: Cytokine and trophic factors from other cells in the retina and tectal extract can promote RGCs to survive, and they can be purified by Thy 1.1 antibody. |
| Keywords: | Cell cultureRetinal ganglion cells |
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