胶体金免疫层析法快速检测鼠疫耶尔森菌F1抗体

目的建立检测鼠疫耶尔森菌F1抗体的胶体金标记免疫层析方法。方法胶体金标记纯化鼠疫F1抗原,双抗原夹心法原理制成免疫层析检测试纸条,并对兔、羊、人和黄鼠血清进行检测评价。结果用80份不同动物种属的阴性血清进行检测,未出现非特异性反应,对5份恢复期病人血清、13份免疫兔血清和2份免疫羊血清检测也取得阳性结果,对于8份I HA临界阳性(滴度1∶20)的达乌尔黄鼠血清检出阳性6份。结论建立了可以用于现场快速检测鼠疫F1抗体的胶体金免疫层析方法。

Rapid detection of the fraction-1 (F1) antibody against Yersinia pestis with colloidal gold-immunochromatography

A method for rapid detection of the fraction 1 (F-1) antibody against Yersinia pestis was developed, in which the purified F-1 antigen was coupled with colloidal gold to be used for the detection of F-1 antibody by means of the double antigen sandwich. The sensitivity, specificity and stability of this method of detection were evaluated by the testing with different sera of animals (rabbits, goats, humans and rattus). A 100% specificity was demonstrated, when 8 IHA-negative rabbit sera, 30 negative Spermophilus dauricus sera observed in Western blot analysis, 4 normal goat sera and 38 normal human sera were assayed with this method. When 8 IHA positive S.dauricus sera (titer: 1∶20) were assayed, 6 positive results were obtained. In addition, positive results were also obtained from 13 immunized rabbit sera, 2 immunized goat sera and 5 human sera. It is concluded a simple and rapid colloidal gold-immunochromatography assay for the detection of F-1 antibody against Y.pestis is established in the present study.