人原发性肝细胞癌核基质蛋白的研究

目的比较正常肝与原发性肝细胞癌（ＨＣＣ）核基质蛋白的异同,观察是否有ＨＣＣ特异性核基质蛋白的存在。方法用双向电泳方法比较了３例正常肝和８例ＨＣＣ核基质蛋白成分。结果正常肝和ＨＣＣ的核基质蛋白组成极为相似,但在ＨＣＣ中发现至少有４个ＨＣＣ的特异性核基质蛋白。其中以分子量为６２０００、等电点为５．３的蛋白最具代表性,它存在于所研究的８例ＨＣＣ中。其余３个ＨＣＣ特异性蛋白亦存在于大多数ＨＣＣ中。３例正常肝组织中未见有这４个ＨＣＣ特异性核基质蛋白。结论ＨＣＣ确实有ＨＣＣ特异性核基质蛋白的存在,它的发现可能会为ＨＣＣ的发生、发展、发病机理的研究提供一个新途径。

Nuclear matrix protein pattern in human hepatocellular carcinoma

Objective To compare nuclear matrix proteins of normal liver with those of hepatocellular carcinoma (HCC) to see whether there are HCC specific proteins. Methods Using high resolution two dimensional polyacrylamide gel electrophoresis, the nuclear matrix proteins of 3 normal livers and 8 HCC were compared. Results There was a high degree of similarity between nuclear proteins of normal liver and those of HCC. Four HCC specific nuclear matrix proteins were identified. Among them, one protein(Mr:62 000; pI:5.3) appeared in all tumor samples. The other three proteins appeared in most of the 8 HCC cases. These proteins were not detected in 3 normal livers. Conclusion There are HCC specific nuclear matrix proteins which may be related to the etiology and pathogenesis of HCC.