| 大肠癌新相关基因HSU17714的染色体定位研究 |
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| 作者姓名： | Cai X Zhang Y Geng L |
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| 作者单位： | 浙江医科大学肿瘤研究所 |
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| 摘 要： | 目的确定大肠癌新相关基因ＨＳＵ１７７１４的染色体定位。方法采用强化荧光原位杂交技术（ＦＩＳＨ）,以生物素化酪胺强化荧光原位杂交信号。结果８０．０％（１２８／１６０）的间期细胞和５９．８％（１０４／１７４）的中期分裂相可见到明显集中的ＨＳＵ１７７１４基因的杂交信号,相应荧光Ｒ带分析中,８５．１％（４０／４７）在２２号染色体上１区３带处有杂交信号。结论ＨＳＵ１７７１４基因定位于２２ｑ１３。
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| 关 键 词： | 结直肠肿瘤 癌基因 染色体 原位杂交 |
Assignment of a novel colorectal cancer-associated gene HSU17714 gene to human chromosome band 22q13 by in situ hybridization |
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| Cai X,Zhang Y,Geng L.Assignment of a novel colorectal cancer-associated gene HSU17714 gene to human chromosome band 22q13 by in situ hybridization[J].Chinese Journal of Oncology,1997,19(3):177-179. |
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| Authors: | Cai X Zhang Y Geng L |
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| Institution: | Cancer Institute, Zhejiang Medical University, Hangzhou. |
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| Abstract: | Objective To identify the chromosomal localization of novel colorectal cancer related gene HSU17714.Methods Enhanced fluorescence in situ hybridization technique was applied with tyramine as the enhancer.Results 80%(128/160) interphase and 60% (104/174) metaphase cells showed obvious hybridization signals of HSU17714.85%(40/47) metaphase cells showed hybridization signals at 22q13 in the fluorescence R banding assay.Conclusion HSU17714 is assigned to human chromosome 22q13. |
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| Keywords: | Colorectal neoplasms Oncogenes Chromosome In situ hybridization |
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