New microsatellite markers for multi-scale genetic studies on Phlebotomus ariasi Tonnoir,vector of Leishmania infantum in the Mediterranean area |
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| Authors: | Jorian Prudhomme Céline Toty Ozge Erisoz Kasap Nil Rahola Baptiste Vergnes Carla Maia Lenea Campino Maria Antoniou Maribel Jimenez Ricardo Molina Arnaud Cannet Bulent Alten Denis Sereno Anne-Laure Bañuls |
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| Institution: | 1. UMR MIVEGEC (IRD 224 – CNRS 5290 – Universités Montpellier 1 et 2), 911 Avenue Agropolis, F34394 Montpellier, France;2. Hacettepe University, Faculty of Science, Department of Biology, Ecology Section, ESRL Laboratories, Beytepe, 0680 Ankara, Turkey;3. Unidade de Parasitologia Médica, Centro de Malária e outras Doenças Tropicais, Instituto de Higiene e Medicina Tropicall (IHMT), Universidade Nova de Lisboa (UNL), Rua da Junqueira, 100, 1349-008 Lisboa, Portugal;4. Laboratory of Clinical Bacteriology, Parasitology, Zoonoses, and Geographical Medicine, Faculty of Medicine, University of Crete, Voutes, Heraklion, 71003 Crete, Greece;5. Unidad de Entomología Médica, Servicio de Parasitología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra, Majadahonda-Pozuelo s/n, Majadahonda, 28220 Madrid, Spain;6. Inserm U1065, Centre Méditerranéen de Médecine Moléculaire, C3M, Université de Nice-Sophia Antipolis, 151, route St Antoine de Ginestière, BP 2 3194, 06204 Nice Cedex, France |
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| Abstract: | The population structure of Phlebotomus ariasi, a proven vector of Leishmania infantum in the Mediterranean area, is still poorly understood. Previously, only two microsatellite loci had been developed to study the population genetics of this species. Herein we use these loci and determined fourteen novel microsatellite loci, useful for the characterization of P. ariasi populations. These loci were tested on three populations of P. ariasi, two from France and one from Portugal. In addition, the usefulness of these markers was also evaluated on seven other sandfly species. |
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| Keywords: | Phlebotomus ariasi Microsatellites Population genetics |
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