双向电泳质谱法研究女性肺腺癌转移淋巴结的蛋白表达

目的:分析肺腺癌转移淋巴结与正常淋巴结组织蛋白质表达差异.方法:运用蛋白质组技术,对肺腺癌转移淋巴结与正常淋巴结组织蛋白质进行分离和比较分析,并在蛋白质和mRNA水平进行进一步验证.结果:获得了背景清晰、分辨率和重复性较好的双向凝胶电泳图谱,比较分析显示与正常淋巴结蛋白质表达水平相比,肺腺癌转移淋巴结有23个蛋白点的表达量存在明显差异.其中新增蛋白点4个,缺失1个,14个蛋白点表达水平明显上调,4个蛋白点表达水平明显下调.共鉴定了11个肺癌转移相关蛋白,其中,候选蛋白膜联蛋白1(ANX1)、细胞骨架蛋白(CK18)、Rho-GDP解离抑制剂1(GDIR)、原肌球蛋白3(TPMF)、蛋白谷氨酰胺γ-谷氨酰转移酶(TGLC)和白介素18(IL-18)在肺腺癌转移淋巴结显著低表达,而候选蛋白核氯离子通道蛋白1(CLI1)、蛋白质二硫键异构酶(ER60)、肌酸激酶、硫氧还蛋白过氧化物酶1(PDX1)和热休克蛋白60(CH60)在肺腺癌转移淋巴结表达水平比正常淋巴结明显提高.结论:肺腺癌转移淋巴结与正常淋巴结蛋白质表达存在明显差异,这11种蛋白质可能与肺腺癌淋巴结转移机制有关.

The research for protein expression of lymph nodes affected by adenocarcinoma of lung in female by 2DE-MS

Objective:To analyze the differential expression of proteome between metastatic lymph nodes in adenocarcinoma of lung and normal lymph nodes. Methods:The proteome techniques were used to separate the protein spots and analyze the differential expression of proteome in the metastatic lymph nodes and normal lymph nodes,and verification was performed at protein and mRNA level,Results:Compared with control,there were 23 protein spots significantly differentially expressed,of which 4 protein spots were newly found,1 protein spot disappeared,14 protein spots increased markedly,and 4 protein spots decreased significantly 11 kinds of the associated protein were identified,among them,ANX1,CK18,Rho-GDP,GDIR,TPMF,TGLC and IL-18 were down-regulated expression significantly in metastatic lymph nodes,but CLI1,ER60,CK,PDX and CH60 were up-regulated expression significantly. Conclusion:The proteomic expression in metastatic lymph nodes of adenocarcinoma of lung is significantly different compared with of the normal control,the 11 kinds of proteins may be associated with the mechanism of metastasis of adenocarcinoma of lung.