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| 应用荧光原位杂交技术检测卵巢浆液性囊腺癌中ZNF217基因拷贝数 | |
| 引用本文： | 钟梅,黎静,丁彦青,宋兰林. 应用荧光原位杂交技术检测卵巢浆液性囊腺癌中ZNF217基因拷贝数[J]. 中华医学遗传学杂志, 2006, 23(6): 665-667 |
| 作者姓名： | 钟梅 黎静 丁彦青 宋兰林 |
| 作者单位： | 1. 510515,广州,南方医科大学南方医院妇产科 2. 510515,广州,南方医科大学南方医院病理科 3. 510515,广州,南方医科大学南方医院血液科 |
| 摘 要： | 目的探讨锌指蛋白217（zinc finger protein 217，ZNF217）基因在卵巢浆液性囊腺癌中20号染色体基因拷贝数改变情况及其临床意义。方法应用荧光原位杂交技术及LSI ZNF217探针对2种卵巢癌细胞株SKOV3、HO-8910和23例卵巢浆液性囊腺癌、10例浆液性囊腺瘤及7份正常卵巢组织进行检测。结果两种卵巢癌细胞株及12例卵巢癌出现ZNF217基因扩增，浆液性囊腺瘤有1例发生了基因的扩增，其余的及正常卵巢组织未出现基因扩增，卵巢癌与卵巢浆液性囊腺瘤、正常卵巢细胞相比较，ZNF217基因拷贝数改变差异有统计学意义（P〈0．05），而低分化的卵巢癌比高分化发生ZNF217基因扩增的几率明显增高（P〈0．05），Ⅲ～Ⅳ期比Ⅰ期的卵巢癌ZNF217基因拷贝数明显增多（P〈0．05）。结论ZNF217基因很可能是卵巢癌发生的促进因子之一，并与卵巢癌分化及预后不良有关。 |
| 关 键 词： | 卵巢癌 荧光原位杂交技术 ZNF217基因 |
| 收稿时间： | 2005-11-30 |
| 修稿时间： | 2005-11-30 |
ZNF217 gene was detected in ovarian serous cystadenocarcinoma by fluorescence in situ hybridization |
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| ZHONG Mei,LI Jing,DING Yan-qing,SONG Lan-lin. ZNF217 gene was detected in ovarian serous cystadenocarcinoma by fluorescence in situ hybridization[J]. Chinese journal of medical genetics, 2006, 23(6): 665-667 | |
| Authors: | ZHONG Mei LI Jing DING Yan-qing SONG Lan-lin |
| Affiliation: | 1 Department of Gynecology and Obstetrics, Nanfang Hospital, Sonthern Medical University, Guangzhou, Guangdong, 510515 P. R. China; 2 Department of Pathology, Nanfanqg Hospital, Sonthern Medical University, Guangzhou, Guangdong, 510515 P. R. China; 3 Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515 P. R. China |
| Abstract: | Objective To investigate amplification of zinc finger protein 217(?ZNF217)gene in ovarian serous cystadenocarcinoma and its clinical significance.Methods Twenty three specimens of ovarian carcinoma,10 specimens of ovarian benign tumors and 7 specimens of normal ovaries and two ovarian cancer cell lines,SKOV3 and HO-8910 were examined by fluorescence in situ hybridization(FISH).Results The amplification of ?ZNF217 was gained in 12 case of ovarian cancers,there was only 1 case in ovarian benign tumor and not amplication in normal ovary.Conclusion The amplification of ?ZNF217 is associated with ovarian cancer.Oncogenes ?ZNF217 maybe play a role in cell differentiation and indicate poorer survival in patients with ovarian cancer. |
| Keywords: | ovarian cancer fluorescence in situ hybridization ZNF217 gene |
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