| 改良的适用于膜片钳研究的大鼠海马神经元急性分离法 |
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| 引用本文： | 笱玉兰,罗利俊,陈国华,朱遂强.改良的适用于膜片钳研究的大鼠海马神经元急性分离法[J].中国康复,2010,25(6):416-418. |
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| 作者姓名： | 笱玉兰 罗利俊 陈国华 朱遂强 |
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| 作者单位： | [1]武汉市第一医院神经内科,武汉430022 [2]华中科技大学同济医学院同济医院神经内科,武汉430030 |
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| 摘 要： | 目的:寻找一种适合膜片钳研究的海马神经元急性分离法。方法:采用出生7～10d的大鼠,用酶机械联合分离法制备海马神经元细胞,用全细胞膜片钳技术对其电生理学特性进行测定。结果:细胞不仅形态学完整,而且具有正常的电生理学特性,能记录到电压依赖性及配体门控GABA电流,还能记录到动作电位。结论:酶机械联合分离法简单快捷,所需酶量少,不需配置混合气,成本低,适合膜片钳的研究。
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| 关 键 词： | 海马神经元 急性分离 膜片钳技术 |
Acute Isolation of Rat Hippocampal Neurons Suitable for Patch-clamping Study |
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| Institution: | GOU Yu-lan,LUO Li-jun,CHEN Guo-hua,et al.Department of Neurology,Wuhan First Hospital,Wuhan 4300220,China |
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| Abstract: | Objective：To find out a single hippocampal neuron suitable for patch-clamp technique.Methods： Acute isolation of hippocampal neurons in 7-10-d-old neonate rats was obtained by enzyme digestion and mechanical separation technique.Electrophysiologic characteristics of hippocampal cells were studied with whole-cell patch-clamp technique.Results： Hippocampal neurons by acute isolation with good characteristics in both morphology and physiology could be obtained.Conclusion： This acute isolation of hippocampal neurons by enzyme digestion and mechanical separation is suitable for the research of patch-clamp technique.This method is simple and cost-saving. |
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| Keywords: | hippocampus neuron acute isolation patch-clamp technique |
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