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| 恙虫病立克次体DNA及DNA序列测定 | |
| 引用本文： | 詹志农,王华民,陶启健,Yu Xue-jie,林碧瑚.恙虫病立克次体DNA及DNA序列测定[J].海南医学院学报,2004,10(1):18-20. |
| 作者姓名： | 詹志农 王华民 陶启健 Yu Xue-jie 林碧瑚 |
| 作者单位： | 1. 海南医学院病原生物学与免疫检测实验室,海口,571101 2. Department of Pathology,University of Texas Medical Branch (UTMB),Galveston of Texas TX77555-0609,USA 3. 海南医学院微生物学教研室,海口,571101 |
| 摘 要： | 目的:检测39例不明原因发热患者血液中恙虫病立克次体DNA。方法:用NPCR检测恙虫病立克次体DNA,取其DNA提取物进行DNA序列分析。结果:序列分析表明扩增的片段为立克次体DNA片段。39例不明原因发热患者血液用NPCR法检出13例恙虫病立克次体DNA。占不明原因发热患者的33.3%。结论:NPCR法可以快速检出恙虫病立克次体DNA,可用于恙虫病的临床早期诊断。 |
| 关 键 词： | 恙虫病 立克次氏体 恙虫热 诊断 化验室 NPCR检测法 |
| 文章编号： | 1007-1237(2004)01-0018-02 |
| 修稿时间： | 2003年7月25日 |
DETERMINATIONS OF DNA AND DNA SEQUENCES OF RICKETTSIA ORIENTALIS |
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| ZHAN Zhi-nong,WANG Hua-min,TAO Qi-jian,et al..DETERMINATIONS OF DNA AND DNA SEQUENCES OF RICKETTSIA ORIENTALIS[J].Journal of Hainan Medical College,2004,10(1):18-20. | |
| Authors: | ZHAN Zhi-nong WANG Hua-min TAO Qi-jian |
| Abstract: | Objective :To detect the Rickecttsia tsutsugamushi DNA from blood specimens of39cases of patients with fever unknown origin ( FUO ) .Method :DNA of Rickecttsia tsutsugamushi was detected by using NPCR and the specific isolation products from the DNA were used for DNA cloning and the DNA sequence analysis.Res ults :DNA sequence analysis confirmed the sequence was the fragment of Rickecttsia tsutsugamushi DNA.13cases of Rickecttsia tsutsugamushi DNA were de鄄tected from all39blood specimens of FUO patients ( 33.33% ) .Conclus ion :It is possible to diagnose clinically the scrub fever early and quickly with NPCR. |
| Keywords: | Tsutsugamushi disease Rickecttsia Tsutsugamushi fever Diagnosis Clinical laboratory NPCR detective method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |