原发性高血压合并脑梗塞患者血管紧张素Ⅱ-1型受体基因多态性的研究

目的 探讨血管紧张素Ⅱ－1型受体（angiotensin Ⅱ type 1 receptor,AT1R)基因A1166／C多态性与原发性高血压及合并脑梗塞的关系。方法 应用聚合酶链反应－限制性片段长度多态性方法检测70名健康人、72例原发性高血压无合并症患者及70例原发性高血压合并脑梗塞患者的AT1R基因型；生化技术测定血脂水平。结果 原发性高血压无合并症组及合并脑梗塞组的C等位基因频率分别为11．1％和10．7％，均显著高于正常对照组的3．6％（P＜0．05）；而两者之间的C等位基因频率差异无显著性（P＞0．05）；牟发性高血压患者血浆脂蛋白a水平与AT1R基因正相关。结论 提示AT1R基因可能是原发性高血压的重要遗传因素，但与高血压病患者是否易患脑梗塞无关。

Influence of angiotensin Ⅱ type 1 receptor gene polymorphism on patients with essential hypertension complicated by brain infarction

OBJECTIVE: To identify the A1166/C polymorphism of angiotensin II type 1 receptor (AT1R) gene in patients with essential hypertension complicated with brain infarction (BI). METHODS: AT1R genotyping with polymerase chain reaction-restrictive fragment length polymorphism was performed in 70 normotensive subjects, 72 hypertensive patients without cardio-cerebrovascular diseases(EH-NCCVD) and 70 hypertensive patients with BI. The relationship between the polymorphism of AT1R gene and plasma lipid levels was also studied. RESULTS: The frequencies of C allele in the two groups of hypertension were higher than that in the health controls, respectively (P<0.05). But there was no significant difference in the frequency of C allele between the two groups of hypertension. A positive correlation was observed between the lipoprotein(a) and AT1R gene A1166/C polymorphism in hypertensive patients. CONCLUSION: AT1R gene contributes to the development of essential hypertensive, but not to the incidence of BI in the hypertensive.