LINC00659靶向miR-149-5p调控食管鳞癌细胞Eca-109的增殖、迁移、侵袭及放射敏感性

目的 探讨长基因间非编码RNA 00659(LINC00659)是否靶向miR-149-5p调控食管鳞癌Eca-109细胞的增殖、 迁移侵袭及放射敏感性.方法 实时定量PCR(RT-qPCR)分析30对食管鳞癌组织、 对照组织中LINC00659和miR-149-5p表达量.将Eca-109细胞分为si-NC组、si-...

LINC00659 Regulates the Proliferation,Migration, Invasion and Radiosensitivity of Esophageal Squamous Cell Carcinoma Eca-109 Cells by Targeting mir-149-5 p

Objective To investigate whether long intergenic non-coding RNA 00659(LINC00659) targets miR-149-5p to regulate the proliferation, migration, invasion and radiosensitivity of esophageal squamous cell carcinoma Eca-109 cells. Methods The expression ofLINC00659 and miR-149-5p in 30 pairs of esophageal squamous cell carcinoma tissues and controltissues was analyzed using real-time quantitative PCR (RT-qPCR). Eca-109 cells cultured in vitrowere divided into si-NC group, si-LINC00659 group, miR-NC group, miR-149-5p group, siLINC00659 + anti-miR-NC group, and si-LINC00659 + anti-miR-149-5p group. The effects ofLINC00659 and miR-149-5p on the proliferation of Eca-109 cells were measured by CCK-8 cell viability assay. The effects of LINC00659 and miR-149-5p on the migration and invasion of Eca-109cells were determined through Transwell migration / invasion assay. The colony formation test wasused to measure the survival fraction and radiosensitization ratio of Eca-109 cells. The relationshipbetween LINC00659 and miR-149-5p was verified by dual luciferase report experiment. Results The expression level of LINC00659 was significantly higher in the esophageal squamous cell carcinoma tissue than in the control group, while the expression level of miR-149-5p was significantly lowerin the esophageal squamous cell carcinoma tissue than in control tissues (P< 0. 05). Compared withthe si-NC group, the proliferation inhibition rate of Eca-109 cells in the si-LINC00659 group wasincreased, and the number of migratory / invasive cells and the cell survival fraction were decreased(P< 0. 05). The radiosensitization ratio of Eca-109 cells in the si-LINC00659 group was 1. 938. Inthe miR-149-5p group relative to the miR-NC group, the proliferation inhibition rate of Eca-109cells was increased, and the number of migratory / invasive cells and the cell survival fraction weredecreased (P< 0. 05). The radiosensitization ratio of Eca-109 cells in the miR-149-5p group was1. 545. miR-149-5p was the target gene of LINC00659. Compared with the si-LINC00659 + anti-miRNC group, the proliferation inhibition rate of Eca-109 cells in si-LINC00659 + anti-miR-149-5pgroup was decreased, and the number of migratory / invasive cells and the cell survival fraction wereincreased (P < 0. 05). The radiosensitization ratio of Eca-109 cells in si-LINC00659 + anti-miR149-5p group was 0. 657. Conclusion The interference of LINC00659 inhibits cell proliferation,migration and invasion, improves cell radiosensitivity of esophageal squamous cell carcinoma Eca109 cells by up-regulating miR-149-5p.