miR-188-3p靶向YAP1抑制肝细胞癌的侵袭和迁移

目的 探究miR-188-3p在肝细胞癌(hepatocellular carcinoma,HCC)中的表达及其抑制HCC细胞侵袭和迁移的分子机制.方法 利用RT-qPCR检测miR-188-3p在人HCC组织和细胞系中的表达情况,进一步分析miR-188-3p表达在HCC中的临床价值;利用Western印迹检测转染m...

miR-188-3p Inhibits the Invasion and Migration of Hepatocellular Carcinoma Cells by Targeting YAP1

Objective To explore the expression of miR-188-3p in hepatocellular carcinoma(HCC ) and its molecular mechanism of inhibiting the invasion and migration of HCCcells. Methods The expression of miR-188-3p in human HCC tissues and cell lines was detectedby RT-qPCR, and the clinical value of miR-188-3p expression in HCC was further analyzed. Western blotting was used to detect the effects of transfection of the miR-188-3p mimics andinhibitor on YAP1 protein in HCC cells. Transwell invasion and migration assay were used to measure the invasive and migratory abilities of cells in each group, respectively. Dual luciferase gene reporting assay was employed to identify the mechanism of miR-188-3p targeting YAP1. Finally, therecovery experiment was performed by co-transfection of miR-188-3p mimics and YAP1 overexpression plasmid in HCC cells. Results The expression level of miR-188-3p was significantly lower inHCC tissues than that in normal liver tissues, and its low expression was closely related to tumorsize, tumor differentiation, and TNM stage ( P < 0. 05, respectively). Further survival analysisshowed that low miR-188-3p expression level in HCC tissues suggested a poor prognosis (log-rankP =0. 025). In vitro cell experiments showed that the overexpression of miR-188-3p significantly inhibited the invasion and migration of HCC cells, while inhibition of miR-188-3p expression showed anopposite effect. Dual luciferase reporter gene assay showed that miR-188-3p could inhibit the expression of YAP1 protein by binding to the 3′UTR of YAP1. The results of the recovery experiment confirmed that miR-188-3p inhibited the invasion and migration of HCC cells by inhibiting the expression of YAP1 protein. Conclusion The expression of miR-188-3p is down-regulated in HCC, andmiR-188-3p inhibits the invasion and migration of HCC cells by targeting the expression of YAP1,thereby participating in the occurrence and progression of HCC.