| 低温下ZF4细胞miR-183-5p的作用研究 |
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| 引用本文： | 牛昊,富伟康,罗军涛,李梦佳,张俊芳,韩兵社. 低温下ZF4细胞miR-183-5p的作用研究[J]. 医学分子生物学杂志, 2022, 19(2): 115-120. DOI: 10.3870/j.issn.1672-8009.2022.02.004 |
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| 作者姓名： | 牛昊 富伟康 罗军涛 李梦佳 张俊芳 韩兵社 |
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| 作者单位： | 上海海洋大学1水产与生命学院,2水产种质资源发掘与利用教育部重点实验室,3水产科学国家级实验教学示范中心 上海市, 2013064上海海洋大学中国科学技术部海洋生物科学国际联合研究中心 上海市, 201306 |
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| 摘 要： | 目的 探讨miR-183-5p在斑马鱼(Danio rerio)胚胎成纤维(ZF4)细胞低温适应中的表达情况与作用机制.方法 RT-qPCR检测ZF4细胞中miR-183-5p的表达,使用靶基因预测数据库Targetscan Fish预测miR-183-5p的靶基因,Turbofect转染试剂对miR-183-5p模拟...
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| 关 键 词： | 斑马鱼 F4细胞 低温 miRNA |
Effect of miR-183-5 p on ZF4 Cells under Low Temperature |
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| NIU Hao,FU Weikang,LUO Juntao,LI Mengjia,ZHANG Junfang,HAN Bingshe. Effect of miR-183-5 p on ZF4 Cells under Low Temperature[J]. Journal of Medical Molecular Biology, 2022, 19(2): 115-120. DOI: 10.3870/j.issn.1672-8009.2022.02.004 |
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| Authors: | NIU Hao FU Weikang LUO Juntao LI Mengjia ZHANG Junfang HAN Bingshe |
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| Affiliation: | 1College of Fisheries and Life Science,2Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education,3 National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China4International Research Center for Marine Biosciences at Shanghai Ocean University, Ministry of Science and Technology, Shanghai, 201306, China |
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| Abstract: | Objective To investigate the expression and mechanism of miR-183-5p in zebrafish(Danio rerio) ZF4 cells during low temperature acclimatization. Methods ZF4 cells were culturedunder 18℃ for 30 days. The expression of miR-183-5p in ZF4 cells was detected by RT-qPCR. Cellviability was determined by CCK-8 cell viability assay after transfection of miR-183-5p mimics/ miRNC with Turbofect cell transfection kit. Targetscan Fish database was used to predict the potentialtarget genes, and dual luciferase activity reporting assay was used to confirm the binding site ofmiR-183-5p and its target gene egr1. Results The expression level of miR-183-5p increased significantly. The conservation analysis showed that miR-183-5p was highly conserved among species. Overexpression of miR-183-5p could enhance the viability of ZF4 cells under low temperature. The potential target genes of miR-183-5p were predicted by bioinformatics methods and tentatively verified by RT-qPCR. The dual luciferase activity reporting assay further confirmed that miR-183-5pcould bind to the 3′UTR of egr1. Conclusion This study demonstrates that miR-183-5p plays a protective role in ZF4 cells during low temperature acclimatization through egr1 in zebrafish. It lays afoundation for further exploration of the mechanisms of zebrafish during low temperature acclimationand provides a new idea for the researches of molecular mechanisms of hypothermia treatment. |
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| Keywords: | zebrafish ZF4 cells low temperature miRNA |
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