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Hydrolysis of adenine nucleotides in platelets from patients with acute myocardial infarction
Authors:Bagatini Margarete D  Martins Caroline C  Battisti Vanessa  Spanevello Roselia M  Gasparetto Diogo  Rosa Cintia S  Gonçalves Jamile Fabrin  Schetinger Maria Rosa C  dos Santos Romualdo B  Morsch Vera Maria
Affiliation:1. Departamento de Química, Centro de Ciências Naturais e Exatas, Programa de Pós-Graduação em Bioquímica Toxicológica, Universidade Federal de Santa Maria, Santa Maria, RS, 97105-900, Brazil;2. Grupo de Pesquisa em Cardiologia, Centro de Ciências da Saúde, Universidade Federal de Santa Maria, RS, Brazil;1. State Key Laboratory of Virtual Reality Technology and Systems, Beihang University, Beijing 100191, China;2. School of Computer and Communication Engineering, Tianjin University of Technology, Tianjin 300384, China;3. Department of Electrical Engineering, The University of Suwon, San 2-2, Wau-ri, Bongdam-eup, Hwaseong-si, Gyeonggi-do 445-743, South Korea;4. Department of Electrical and Computer Engineering, University of Alberta, Edmonton, AB, Canada T6G 2G6;5. Systems Research Institute, Polish Academy of Sciences, Warsaw, Poland;1. Programa de Pós-Graduação em Ciências Biológicas, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil;2. Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas, Campus Universitário-Capão do Leão, 96010-900 Pelotas, RS, Brazil;3. Curso de Enfermagem, Universidade Federal da Fronteira Sul, Campus Chapecó 89812-000, Chapecó, SC, Brazil;1. Biological Sciences Department, Faculty of Science, University of Jeddah, P.O. Box 80203, Jeddah, Saudi Arabia;2. Cell Biology Department, Genetic Engineering and Biotechnology Division, National Research Centre, Dokki, P.O. Box 12622, Gizza, Egypt;3. Department of Chemistry of Natural and Microbial Products, Division of Pharmaceutical and Drug Industries, National Research Centre, Dokki, P.O. Box 12622, Gizza, Egypt;4. King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia;5. Department of Molecular Genetics and Enzymology, Davison of Human Genetics and Genome Research, National Research Centre, Dokki, P.O. Box 12622, Gizza, Egypt;6. Embryonic Stem Cell Unit, King Fahd Medical Research Center, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia;7. Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, P.O. Box 80203, Jeddah, Saudi Arabia;8. Laboratory of Structural Dynamics, Stability and Folding of Proteins, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia;9. Department of Molecular Medicine and USF Health Byrd Alzheimer’s Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA;1. Programa de Pós-Graduação em Ciências Biológicas: Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Campus Universitário, Camobi, 97105-900 Santa Maria, RS, Brazil;2. Universidade Federal de Mato Grosso, Conselho de Ensino e Pesquisa, Curso de Medicina. Parque Sagrada Família, Rondonópolis, MT, Brazil;3. Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, Campus Bento Gonçalves, RS, Brazil;4. Universidade Federal da Fronteira Sul, Campus Chapecó, Chapecó, SC, Brazil;5. Universidade Luterana do Brasil—Campus Carazinho, RS, Brazil;1. Departament of Biochemistry and Molecular Biology, Federal University of Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil;2. Departament of Microbiology and Parasitology, Federal University of Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil;3. Laboratory of Experimental Surgery, Federal University of Santa Maria, Av. Roraima, 97105-900 Santa Maria, RS, Brazil;4. Department of Biochemistry, Federal University of Technology, P. M. B. 704, Akure 340001, Nigeria
Abstract:
ObjectivesTo investigate the rate of ATP, ADP and AMP hydrolysis on the surface of platelets from acute myocardial infarction (AMI) patients.Design and methodsTwenty-five patients diagnosed with AMI, through clinical criteria, electrocardiographic changes and increase of cardiac biomarkers, as well as 25 healthy patients were selected. The hydrolysis of ATP, ADP and AMP was verified in isolated platelets of these patients.ResultsThe results demonstrated that an increase in ATP (54%) and ADP (45%) hydrolysis occurred in AMI patients when compared to the control group. The hydrolysis of AMP also increased by 46% in AMI patients probably leading to an enhancement in the adenosine level.ConclusionsOur results suggest an increase in nucleotide hydrolysis in platelets from AMI patients, which could be related to a compensatory organic response to thrombotic events that occur in AMI.
Keywords:
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