| 香加皮水提取物诱导人胃癌细胞BGC-823凋亡及其作用机制 |
| |
| 引用本文： | 单保恩,李俊新,张静. 香加皮水提取物诱导人胃癌细胞BGC-823凋亡及其作用机制[J]. 中草药, 2005, 36(8): 1184-1188 |
| |
| 作者姓名： | 单保恩 李俊新 张静 |
| |
| 作者单位： | 河北医科大学第四医院,科研中心,暨河北省肿瘤基因诊断、预防和治疗重点实验室,河北,石家庄,050011 |
| |
| 基金项目： | 国家自然科学基金资助项目(30371753),河北省自然科学基金资助项目(C2004000610) |
| |
| 摘 要： | 目的研究香加皮水提取物(CPE)诱导人胃癌细胞BGC-823凋亡及其作用机制。方法采用G iem sa染色观察细胞凋亡形态学变化;电子显微镜观察凋亡细胞的超微结构变化;流式细胞术和琼脂糖凝胶电泳方法检测BGC-823细胞凋亡率、细胞周期和细胞凋亡的DNA水平变化;RT-PCR方法检测细胞凋亡相关基因bcl-2、bax和surv iv in mRNA表达水平变化;免疫细胞化学方法检测bcl-2、bax和surv iv in蛋白表达的变化。结果经CPE作用后,人胃癌细胞BGC-823出现明显的细胞凋亡形态学变化及超微结构改变,细胞DNA琼脂糖凝胶电泳呈现梯形图。经250μg/mL CPE处理48 h后,多数BGC-823细胞被阻滞在G2/M期,而且细胞发生明显的凋亡变化,BGC-823细胞凋亡率可达18.9%。CPE可抑制BGC-823细胞bcl和surv iv in mRNA及蛋白的表达,促进baxmRNA及蛋白的表达。CPE可明显延长S180荷瘤小鼠生存期,且具有剂量依赖性。结论CPE通过阻滞BGC-823细胞于G2/M期及诱导BGC-823细胞凋亡发挥抗肿瘤作用,其作用机制与抑制细胞的bcl-2和surv iv in基因mRNA及蛋白表达、促进bax基因和蛋白的表达有关。
|
| 关 键 词： | 香加皮 人胃癌细胞BGC-823 bcl-2 bax survivin |
| 文章编号： | 0253-2670（2005）08-1184-05 |
| 收稿时间： | 2004-12-22 |
| 修稿时间： | 2004-12-22 |
Inductive effect of Cortex Periplocae extract on apoptosis of human gastric cancer cells BGC-823 |
| |
| SHAN Bao-en,LI Jun-xin and ZHANG Jing. Inductive effect of Cortex Periplocae extract on apoptosis of human gastric cancer cells BGC-823[J]. Chinese Traditional and Herbal Drugs, 2005, 36(8): 1184-1188 |
| |
| Authors: | SHAN Bao-en LI Jun-xin ZHANG Jing |
| |
| Affiliation: | Hebei Key Laboratory of Gene Diagnosis, Prophylaxis, and Therapy, Research Center, The Fourth Hospitalof Hebei Medical University, Shijiazhuang 050011, China;Hebei Key Laboratory of Gene Diagnosis, Prophylaxis, and Therapy, Research Center, The Fourth Hospitalof Hebei Medical University, Shijiazhuang 050011, China;Hebei Key Laboratory of Gene Diagnosis, Prophylaxis, and Therapy, Research Center, The Fourth Hospitalof Hebei Medical University, Shijiazhuang 050011, China |
| |
| Abstract: | Objective To study the inductive effect ofCortex Periplocae extract(CPE)on apoptosis of human gastric cancer cells BGC-823 and its mechanism.Methods The cell morphology and super-microstructural changes of apoptosis were analysed by Giemsa stain-ing and electric microscope,respectively.The BGC-823 apoptosis ratio,cell cycles,and changes of apoptosis in DNA level were studied by Flow Cytometry and agarose gel electrophoresis.The genes mRNA and protein expression of apoptosis-related genes bcl-2,bax,and survivin were studied by RT-PCR and immunology cell chemistry method.Results After treatment with CPE,BGC-823 cells showed some typical morphologic features and super-microstructural changes of apoptosis.DNA agarose gel electrophoresis showed characteristic"DNA ladder" pattern.Most BGC-823 cells were arrested at G2/M phase.Some typical subdiploid peaks before G0/G1 phase were observed.The apoptotic rate of BGC-823 was 18.9%after 250 tlg/mL CPE treated for 48 h.The gene mRNA and protein expression of bcl-2 and survivin were inhibi-ted by CPE,whereas that of bax was up-regulated.CPE could enhance the 1ife span of S180 bearing mice in a dose-dependent manner.Conclusion CPE can inhibit the tumor growth by arresting the BGC-823 cell cycle at G2/M phase and inducing BGC-823 apoptosis.Its mechanism iS related to the inhibition on gene mRNA and protein expression of bcl-2 and survivin,and enhancement of those of bax. |
| |
| Keywords: | Cortex Periplocae human gastric cancer cells BGC-823 bcl-2 bax survivin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中草药》浏览原始摘要信息 |
|
点击此处可从《中草药》下载全文 |
|
