| TaqMan探针检测结直肠癌血浆中微量甲基化EphA7基因 |
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| 引用本文： | 王建东,王绪林,董迎春,李国立,鲍杨,马恒辉,周晓军. TaqMan探针检测结直肠癌血浆中微量甲基化EphA7基因[J]. 东南国防医药, 2009, 11(2): 97-99,130 |
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| 作者姓名： | 王建东 王绪林 董迎春 李国立 鲍杨 马恒辉 周晓军 |
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| 作者单位： | 1. 南京军区南京总医院病理科,江苏南京,210002
2. 南京军区南京总医院普外科,江苏南京,210002 |
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| 基金项目： | 国家重点基础研究发展规划(973计划)，南京军区医学科学技术研究计划重点课题 |
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| 摘 要： | 目的从结直肠癌症患者血浆中分离微量来源于肿瘤细胞的游离DNA，检测甲基化EphA7作为肿瘤标志。方法提取血浆中微量游离DNA，并以亚硫酸氢钠修饰DNA。根据修饰后的DNA序列，设计EphA7甲基化特异性引物和探针，利用Real Time PCR仪扩增甲基化EphA7基因。结果从大肠癌患者血浆中检出微量甲基化EphA7基因。结论应用荧光标记TaqMan探针Real Time PCR可以检测出结直肠癌患者血浆中微量游离DNA中甲基化EphA7基因，血浆甲基化EphA7可能作为一种新的肿瘤标志物。
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| 关 键 词： | 荧光标记探针 实时PCR 血浆 甲基化 EphA7 |
Microamount of hypermethylated EphA7 detected in plasma of colorectal cancer with a TaqMan probe |
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| WANG Jian-dong,WANG Xu-lin,DONG Ying-chun,LI Guo-li,BAO Yang,MA Heng-hui,ZHOU Xiao-jun. Microamount of hypermethylated EphA7 detected in plasma of colorectal cancer with a TaqMan probe[J]. Journal of Southeast China National Defence Medical Science, 2009, 11(2): 97-99,130 |
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| Authors: | WANG Jian-dong WANG Xu-lin DONG Ying-chun LI Guo-li BAO Yang MA Heng-hui ZHOU Xiao-jun |
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| Affiliation: | 1.Department of Pathology;2.Department of Surgery;Nanjing General Hospital of Nanjing Military Command;PLA;Nanjing 210002;Jiangsu;China |
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| Abstract: | Objective To detect hypermethylated EphA7 in plasma DNA released from colorectal cancer cells as a tumor marker.Methods The free DNA in plasma was extracted using a QIAamp DNA Blood Mini kit and treated with sodium bisolfite.The Real time PCR primer sets and fluorescence labeled probe for detection of hypermethylated EphA7 were designed according to the sodium bisulfte converted DNA sequence.Hypermethylated EphA7 DNA was detected using an ABI PRISM 7000 sequence detection system.Results The hypermethylated ... |
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| Keywords: | EphA7 |
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