| 献血者血液中HCMVpp65检测方法的建立与初步应用 |
| |
| 引用本文： | 刘衍春,刘洪莉,刘毅,唐荣才,盛大祥. 献血者血液中HCMVpp65检测方法的建立与初步应用[J]. 中国实验血液学杂志, 2004, 12(4): 528-530 |
| |
| 作者姓名： | 刘衍春 刘洪莉 刘毅 唐荣才 盛大祥 |
| |
| 作者单位： | 江苏省血液中心输血研究室,南京,210042 |
| |
| 摘 要： | 为保证病人的用血安全 ,建立适合于血站对HCMV病原检测的方法 ,本研究采用免疫组织化学的技术 ,结合本实验室具体条件检测HCMV的 pp6 5 ,细胞计数 (6 - 8)× 10 6/ml,取量 2 0 μl,涂片油镜镜检5 0 0 0 0个细胞。结果表明 :在 10 3人份样本中 ,阳性标本为 10例 ,阳性率为 9.71% ,对 10名阳性人中的 2人进行第 2次pp6 5的监测 ,结果均为阳性。结论 :该方法简单、快速、有效 ,完全适合于血站系统对献血者的HCMV进行病原学检测 ,有推广的价值。
|
| 关 键 词： | 献血者 HCMVpp65 检测 |
| 文章编号： | 1009-2137(2004)04-0528-03 |
| 修稿时间： | 2003-09-30 |
Establishment of Detection Method for HCMVpp65 of the Blood Donors and Its Application in Blood Bank |
| |
| LIU Yan-Chun,LIU Hon-li,LIU Yi,TANG Rong-Cai,SHENG Da-Xiang Jiangsu Province Blood Center,Nanjing ,China. Establishment of Detection Method for HCMVpp65 of the Blood Donors and Its Application in Blood Bank[J]. Journal of experimental hematology, 2004, 12(4): 528-530 |
| |
| Authors: | LIU Yan-Chun LIU Hon-li LIU Yi TANG Rong-Cai SHENG Da-Xiang Jiangsu Province Blood Center Nanjing China |
| |
| Affiliation: | Jiangsu Province Blood Center, Nanjing 210042, China. |
| |
| Abstract: | To establish method suitable to assay HCMVpp65 of the blood donors in blood bank and to supply safe blood to the patients, the immunocytochemical techniques were used, (6 - 8) x 10(6)/ml cells were counted, 50 x 10(3) cells were detected by light microscope, The results showed that 10 positive samples in 103 samples were found, positive rate was 9.71%, among 10 positive samples, 2 samples were still positive in the second detecting. In conclusion, this method is simple, quick and effective, suitable to detect HCMVpp65 of the blood donors in the blood bank. |
| |
| Keywords: | Blood donor HCMVpp65 detection |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
