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铁皮石斛F1代田间试验与优良家系选择
引用本文:章晓玲,斯金平,吴令上,郭英英,俞洁,王林华. 铁皮石斛F1代田间试验与优良家系选择[J]. 中国中药杂志, 2013, 38(22): 3861-3865
作者姓名:章晓玲  斯金平  吴令上  郭英英  俞洁  王林华
作者单位:浙江农林大学 亚热带森林培育国家重点实验室培育基地 浙江省铁皮石斛产业技术创新战略联盟, 浙江 临安 311300;浙江农林大学 亚热带森林培育国家重点实验室培育基地 浙江省铁皮石斛产业技术创新战略联盟, 浙江 临安 311300;浙江农林大学 亚热带森林培育国家重点实验室培育基地 浙江省铁皮石斛产业技术创新战略联盟, 浙江 临安 311300;浙江农林大学 亚热带森林培育国家重点实验室培育基地 浙江省铁皮石斛产业技术创新战略联盟, 浙江 临安 311300;奉化市九味圣草堂铁皮石斛专业合作社, 浙江 奉化 315500;浙江佳诚生物工程有限公司, 浙江 义乌 322022
基金项目:浙江省重大科技专项(2012C12912,2011C12002);宁波市农业创新重点项目
摘    要:
基于随机区组试验设计,对14个铁皮石斛F1代家系进行遗传测定,结果表明不同家系间农艺性状和产量均存在显著差异,杂交优势明显;筛选出6a×2b,9×66,78×69共3个优良家系在产量、农艺性状与产品专用化等方面具有明显的优势。农艺性状与生物产量、经济产量相关性分析表明,株高、茎粗、茎节数量、节间距、叶片数量、叶片长度、叶片宽度等农艺性状与生物产量、经济产量存在显著相关性,其中茎粗、叶片数量和叶片长度对生物产量、经济产量影响最为显著,并能建立最优线性回归模型;萌蘖数少于4.5株/丛时,生物产量、经济产量均会随萌蘖数的增加而增加,但大于4.5株/丛时,萌蘖数对生物产量、经济产量影响不大。二年生与一年生主要农艺性状比较,萌蘖数、茎粗和叶形指数基本稳定,可用于早期 选择。

关 键 词:铁皮石斛  种内杂交  田间试验  农艺性状  产量
收稿时间:2013-03-13

Field experiment of F1 generation and superior families selection of Dendrobium officinale
ZHANG Xiao-ling,SI Jin-ping,WU Ling-shang,GUO Ying-ying,YU Jie and WANG Lin-hua. Field experiment of F1 generation and superior families selection of Dendrobium officinale[J]. China Journal of Chinese Materia Medica, 2013, 38(22): 3861-3865
Authors:ZHANG Xiao-ling  SI Jin-ping  WU Ling-shang  GUO Ying-ying  YU Jie  WANG Lin-hua
Affiliation:Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China;Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China;Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China;Nurturing Station for State Key Laboratory of Subtropical Silviculture, Zhejiang Agricultural and Forestry University, Zhejiang Provincial Strategic Alliance for Technical Innovation in Industry of Dendrobium officinale, Lin'an 311300, China;Fenghua Jiuwei Shengcaotang Professinal Cooperatives of Dendrobium officinale, Fenghua 315500, China;Zhejiang Jiacheng Biological Engineering Co., Ltd., Yiwu 322022, China
Abstract:
Based on randomized block design of experiment, agronomic traits and yields of 14 F1 generations of Dendrobium officinale were determined. The results showed that the differences in agronomic traits and yields among families were significant, and the hybrid vigor was obvious. Families of 6b×2a, 9×66 and 78×69 were selected with the remarkable superiority of yields, agronomic traits and product customization. Correlation analysis between agronomic traits and yields showed that plant height, stem diameter, leaf number, blade length and blade width were all significantly correlated with biological yields and economic yields. Among which, stem diameter, leaf number and blade length were the most significant, and an optimal linear regression model could be established. When the number of shoots was fewer than 4.5, both biological yields and economic yields increased with the increasing number of shoots, but it could not much affect yields when the number of shoots was larger than 4.5. Shoots number, stem diameter and leaf index were basic stability when compared biennial traits to annual, which could be used for early selection.
Keywords:Dendrobium officinale  intraspecific hybridization  field experiment  agronomic traits  yields
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