槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响 |
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引用本文: | 严梅娣,叶孟,林蕾,倪曙民,王少敏,刘素荣.槲寄生凝集素腹腔内给药对腹腔巨噬细胞功能的影响[J].中国临床药理学与治疗学,2010,15(2):126-129. |
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作者姓名: | 严梅娣 叶孟 林蕾 倪曙民 王少敏 刘素荣 |
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作者单位: | 1. 宁波市第七医院普外科,宁波315202,浙江 2. 宁波大学医学院附属医院,宁波315000,浙江 |
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基金项目: | 浙江省自然基金项目,宁波市博士基金项目,宁波市医学科研项目 |
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摘 要: | 目的:探讨小鼠腹腔内注射槲寄生凝集素对腹腔巨噬细胞功能的影响。方法:16只小鼠分为腹腔内给药组和空白组,分别取腹腔内巨噬细胞并计数,采用ELISA法测定TNF-α表达情况,Griess法测定NO表达,MTT法检测腹腔内巨噬细胞对肠癌HCT116细胞的杀伤作用。结果:腹腔内给槲寄生凝集素后可明显增加腹腔内巨噬细胞数,并增加TNF—α和NO的表达水平;激活的巨噬细胞对HCT116细胞的杀伤作用增强,其杀伤作用与TNF—α和NO表达时间一致。结论:腹腔内注射槲寄生凝集素可激活腹腔内巨噬细胞,激活的巨噬细胞对HCT116细胞有杀伤作用。
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关 键 词: | 结直肠肿瘤 槲寄生凝集素 巨噬细胞 腹腔内化疗 |
Effect of Mistletoe Lectins on immunological roles of peritoneal macrophages by intraperitoneal administration |
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Institution: | YAN Mei-di , YE Meng, LIN Lei , NI Shu-min , WANG Shao-min , LIU Shu-rong(1 Department of Surgery, Ningbo Seventh Hospital, Ningbo 315002, Zhejiang, China; 2 The Af filiated Hospital of Medical School of Ningbo University, Ningbo 315000, Zhejiang,China) |
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Abstract: | To investigate the effect of peritoneal macrophages in mice on tumoricidal activity against colon cancer HCT116, following intraperitoneal injection of Mistletoe Lectins. METHODS: 16 mice were divided into control group and intraperitoneal chemotherapy group. The number of peritoneal macrophages was counted by trypan blue exclusion. The amount of TNF-α and NO expressed by peritoneal macrophages was measured by ELISA and Griess, respectively. The cytotoxity of peritoneal macrophages supernatant against HCT116 was evaluated by MTT assay. RESULTS: It was found that the amount of peritoneal macrophages was increased, the expressions of TNF-a and NO were increased and the cytotoxicity against HCT116 in vitro was increased after intraperitoneal injection of Mistletoe Lectins. CONCLUSION: Intraperitoneal injection of Mistletoe Lectins can activate the peritoneal macrophages in mice and enhance the cytotoxity of the supernatant against HCT116 in vitro. |
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Keywords: | Colorectal neoplasm Mistletoe Lectins Macrophage Intraperitoneal chemotherapy |
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