食管癌患者化疗前后ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞及Ｆｏｘｐ３ｍＲＮＡ的表达及其临床意义

目的：探讨晚期食管癌患者化疗前后外周血中ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞及Ｆｏｘｐ３ｍＲＮＡ的表达变化及其临床意义。方法：采用流式细胞术检测６８例晚期食管癌患者化疗前后外周血ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞的水平，并与４０例健康成人进行比较。同时运用ＲＴ-ＰＣＲ方法检测其中４０例患者化疗前后外周血调节性Ｔ细胞Ｆｏｘｐ３基因ｍＲＮＡ的表达情况。结果：（１）食管癌患者外周血中ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞占ＣＤ４＋Ｔ细胞总数的（１.８２±０.５４）％，显著高于健康对照组的（１.５２±０.７０）％（Ｐ＜０.０１）；（２）化疗前患者外周血中ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞为（１.８２±０.５４）％，明显高于化疗后的（１.６６±０.５８）％（Ｐ＜０.０5）；（３）化疗前患者调节性Ｔ细胞Ｆｏｘｐ３基因ｍＲＮＡ的相对表达量为０.３１８±０.０２７，明显高于化疗后的０.２６６±０.０２８（Ｐ＜０.０５）。结论：食管癌患者接受化疗后外周血中Ｆｏｘｐ３ｍＲＮＡ水平与ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞数量表达均显著下降，推测Ｆｏｘｐ３基因可能对ＣＤ４＋ＣＤ２５ｈｉｇｈＴ细胞有重要的调节功能，从而影响食管癌患者化疗后ＣＤ４＋ＣＤ２５ｈｉｇｈ调节性Ｔ细胞的水平。

The expression and clinical significance of CD4+CD25high regulatory T cells and Foxp3 mRNA in advanced esophageal cancer patients pre-treated and treated with chemotheraphy

Objective：To explore the changes of CD4＋CD25high regulatory T cells and Foxp3 mRNA in patients with advanced esophageal cancer and its clinical significance. Methods：The frequency of CD4＋CD25high regulatory T cells in peripheral blood of 68 patients with advanced esophageal cancer and 40 healthy controls was determined by flow cytometry, the expression of Foxp3 mRNA in these regulatory T cells of 40 patients with advanced esophageal cancer were detected by RT-PCR. Results：CD4＋CD25high regulatory T ceils numbers were significantly more in 68 patients with advanced esophageal cancer （ CD4＋ T cells） than in 40 healthy donors. The level of CD4＋CD25high T cells was more in advanced esophageal cancer patients treated with chemotheraphy than in those patients with- out chemotheraphy. The level of Foxp3 mRNA were significantly less in advanced esophageal cancer patients treated with chemotheraphy than in those patients without chemotheraphy. Conclusion：As the level of CD4＋CD25highT cells, the level of Foxp3 mRNA significantly droped in advanced esophageal cancer patients treated with chemotheraphy, and Foxp3 genes may play a pivotal role in regulating the function of Treg cells and the level of CD4＋CD25highT in advanced esophageal cancer patients treated with chemotheraphy.