| 一种可同时显示三种粘液的粘液套染法 |
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| 引用本文： | 王敏,唐志姣,夏东. 一种可同时显示三种粘液的粘液套染法[J]. 武汉大学学报(医学版), 2001, 22(3): 285-287 |
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| 作者姓名： | 王敏 唐志姣 夏东 |
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| 作者单位： | 武汉大学医学院病理学教研室,;武汉大学医学院病理学教研室,;武汉大学医学院病理学教研室, |
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| 摘 要： | 目的 :探讨一种能在同一张切片中同时显示硫酸化酸性粘液、非硫酸化酸性粘液及中性粘液的粘液染色法。方法 :将高铁二胺 (HID)、爱先蓝 (AB)及过碘酸雪夫氏反应 (PAS)三种粘液染色法结合起来 ,按一定顺序 ,对14 7例胃粘膜肠化及胃癌标本中的粘液进行套染 ,并提出HID AB(pH2 .5 ) PAS粘液套染法。结果 :硫酸化粘液呈棕黑色 ,一般酸性粘液呈蓝色 ,中性粘液呈红色。在同一张切片中三种粘液颜色有明显反差 ,背景清晰。结论 :HID AB(pH2 .5 ) PAS粘液套染法是一种便捷、实用、值得推广的粘液染色法
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| 关 键 词： | 染色法 粘液 组织化学 |
| 修稿时间： | 2000-06-27 |
A Triple Staining Manifesting Three Kinds of Mucus on the Same Section |
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| Wang Min,Tang Zhi jiao,Xia Dong. A Triple Staining Manifesting Three Kinds of Mucus on the Same Section[J]. Medical Journal of Wuhan University, 2001, 22(3): 285-287 |
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| Authors: | Wang Min Tang Zhi jiao Xia Dong |
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| Affiliation: | Wang Min,Tang Zhi jiao,Xia Dong Department of Pathology,Medical College,Wuhan University,Wuhan 430071,China |
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| Abstract: | Objective: To investigate a triple staining of acid sulfmucus, acid non sulfmucus and neutromucus in one section. Methods: High iron diamine (HID) , alcian blue(AB) combined with periodic acid shiff (PAS) stainings were used in turn to observe different tinctions of 54 gastric carcinomas and 93 intestinalizations of gastromucosa. Results: The colors were brown black in sulfmucous , blue in non sulfmucus and red in neutromucus, which had obvious contrast and clear background in every section. Conclusion: The HID AB(PH2.5) PAS mucus staining is a convenient and useful method which is worthy of popularization. |
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| Keywords: | staining mucus histochemistry |
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