附子不同炮制品及其部位急性毒性研究

目的:比较中药附子不同的炮制品及其部位的急性毒性差异.方法:采用阶梯法对附子4种炮制品及部分活性成分的LD50或最大耐受量的测定,对不同附子炮制品及活性成分的毒效进行评价.结果:生附片水提液、醇提液的LD50依次为22.4 g/kg、13.2 g/kg；白附、黑附、炮附片醇提液的LD50为131.7 g/kg、44.8g/kg、254.3g/kg;附子中脂溶性生物碱的LD50为0.5 mg/kg；白附、黑附、炮附片的最大耐受量依次为533 g、666 g、266 g；附子水溶性生物碱和附子多糖的最大耐受量依次为161 g、533 g.结论:不同炮制方法得到的附子及其部位药理活性差异较大,毒效在

Acute Toxicity of Different Processed Products and its Parts of Aconitum Carmichalii Debx

Objective： Compare the difference between acute toxicity of different processed products and its parts of Aconitum Carmichalii Debx. Methods： Use the staircase method to determine the LDs0 or maximal tolerance dose of four kinds of processed prod- ucts and part of active ingredient of Aconitum Carmichalii Debx, to evaluate tile toxic-effect of different processed products and part of active ingredient of Aconitum Carmichalii Debx. Results： The LDs0 of water extract, alconol extract of Radix aconite praeparata are 22.4 g/kg, 13.2 g/kg; The LDso of alconol extract of white Radix aconite praeparata, black Radix aconite praeparata, processed Radix aconite praeparata are 151.7 g/kg, 44. 8 g/kg, 254. 3 g/kg; The LDso of fat-soluble alkaloid of Aconitum Carmichalii Debx is 0. 5 mg/kg ; The maximal tolerance dose of white Radix aconite praeparata, black Radix aconite praeparata, processed Radix aconite praeparata are 533 g, 666 g, 266 g; The maximal tolerance dose of water-soluble alkaloid of Aconitum Carmichalii Debx and polysac- charide of Aconitum Carmichalii Debx are 161 g, 533 g. Conclusion： The large difference on the different processing methods to ob- tain Aconitum Carmichalii Debx and Pharmacological Activities of its parts, the toxic-effect show difference in the processed products of Aconitum Carmichalii Debx and its part of active ingredient.