二种纯系小鼠动物模型应用于布鲁氏菌病传染免疫的研究

以细菌学、血清学、细胞免疫学及病理组织学等方法,较系统地应用牛、羊和猪布鲁氏菌毒种建立二个纯系小鼠(BALB/c及C57/BL)布鲁氏菌病动物模型。发现BALB/c感染布鲁氏菌的过程较明显,尤其用牛种544A皮下感染时更为明显,表明BALB/c小鼠可作为慢性感染动物模型。抗体形成细胞(PFC)的动态观察表明,三种布鲁氏菌对小鼠PFC的形成均有不同程度的抑制作用,其中以羊种布鲁氏菌的抑制作用最为明显。用布氏菌素进行皮内变态反应的观察发现,BALB/c小鼠对布氏菌素的皮内变态反应不敏感;而C57/BL小鼠则较为敏感,但在感染后第45天左右有一免疫抑制阶段。本研究为国内外广泛利用纯系小鼠作为布鲁氏菌传染免疫的研究提供了基础。

Preliminary Study of Two Inbred Mice Models for Infection and Immunity of Brucellosis

We established 2 animal models of Brucellosis using inbred mice (BALB/c and C57/BL) infected -with virulent strains of B. melitensis 16, B. abortus 544 and B. suis 12 followed by bacteriological, serological, (cellular immun- ological as well as histopathological 'examinations. It was shown that the BALB/c was more sensitive than C57/BL to brucella infection, especially to B. abortus 544. It is suggested that BALB/c infected with 544A might be the experimental animal of choice for studying chronic brucella infection. Studies on PFC kinetics of murine Brucellosis were undertaken. It was found that there were varying degrees of immunosuppression of PFC formation in the infectious process of three species of br'ucella, particularity in B. mel-itensis 16 M infection. We also observed the allergic reaction of the infected mice to intracutaneous administration of brucellin and found that . the C57/BL was more sensitive than BALB/c. It was shown that there was an immunosuppression period between the 37th-45th days after infection in C57/BL. The above mentioned findings provide elementary data for study on the role of immunosuppression in the pathogenesis of Brucellosis. It also provides some basic data for the study of experimental brucella infection in inbred mice.