藏药俄色不同提取物降血糖作用比较

目的: 探讨藏药俄色3种提取物对实验性糖脲病小鼠的影响。 方法: 建立链脲佐菌素(STZ,150 mg·kg^-1 ip)诱导小鼠1型糖尿病模型和四氧嘧啶(ALX, 200 mg·kg^-1 ip)诱导小鼠1型糖尿病模型,分别灌胃俄色黄酮(180 mg·kg^-1)、俄色浸膏(900 mg·kg^-1)和康珠甘露(360 mg·kg^-1),连续给药4 d,于每次给药1 h后测定小鼠空腹血糖,并在试验第4天测定血糖后测定小鼠胸腺和肝脏指数,测定血清胰岛素和C肽含量。 结果: 与正常组比较,模型组空腹血糖值明显升高、血清胰岛素和C肽含量明显降低、胸腺指数明显降低、肝脏指数明显升高(P<0.01);与模型组比较,3种提取物均能在一定程度上改善两个糖尿病模型小鼠的一般状态,明显降低空腹血糖和升高血清胰岛素、C肽含量(P<0.05,P<0.01);俄色浸膏、康珠甘露还能降低肝脏指数(P<0.05,P<0.01)。 结论: 俄色3种提取物均有一定降血糖作用,以俄色浸膏作用较好,机制有待进一步研究。

Effects of Three Different Extracts from Malus toringoides in Experimental Diabetic Mice

Objective: To investigate the effects of three different extracts from Malus toringoides (E Se)on experimental diabetic mice. Method: The mice were intraperitoneally injected with streptozocim(STZ,150 mg·kg^-1) or alloxan(ALX),200 mg·kg^-1) to establish diabetic mice. Then the diabetic mice were administrated with E Se flavonoid(180 mg·kg^-1),E Se Jingao(900 mg·kg^-1)and Kangzhu Ganlu(360 mg·kg^-1) by intragastric in grouped mice respectively once daily for 4 days. All mice were fasted for 5 h before administration and the fasting blood glucose (FBG) was tested 1h after treatment. Body weight was determined by every other day. At the end of treatment, the mice were sacrificed in fasting condition after the FBG detection. The blood was collected for insulin and c-peptide detection. Once blood had been collected, the thymus and liver were immediately removed, individually weighed and expressed in relative terms. Result: Compared with the control group, the general behavior of mice treated with extracts from E Se became better and the FBG levels were significantly reduced(P<0.05). Significant increase in serum insulin (P<0.05) and decrease in c-peptide were detected in treated mice(P<0.05). The group of E Se Jingao and Kangzhu Ganlu showed a significant decrease in liver, relative masses compared with model group(P<0.05). Conclusion: The three different extracts from E Se all have a high hypoglycemic activity, further investigations are needed to explore the exact mechanism of action for antidiabetic effect.