鹿茸健骨滴丸对骨质疏松症大鼠肾组织TRPV5表达的影响

目的: 通过观察鹿茸健骨滴丸对糖皮质激素性骨质疏松症(GIO)大鼠股骨骨密度(BMD)及肾组织钙转运通路蛋白(TRPV5) mRNA和蛋白表达的影响,探讨GIO的病理机制及鹿茸健骨滴丸的疗效。 方法: 将82只Wistar大鼠随机分为正常对照组(A组)、模型组(B组)、鹿茸健骨滴丸组(C组) 及骨疏康组(D组)。采用后肢肌注地塞米松(2.5 mg·kg^-1,每周2次)造模,造模同时,C,D 组分别按0.88, 2.1 g·kg^-1·d^-1 剂量ig给药,造模及给药9周后,应用XR-26型双能X射线骨密度仪测定BMD,采用实时定量PCR法及Western blot法测定肾组织TRPV5 mRNA和蛋白表达。 结果: 与正常组比较,模型组大鼠BMD明显降低(P<0.01),肾组织TRPV5 mRNA与蛋白表达均明显下降(P<0.01);与模型组比较,鹿茸健骨滴丸组BMD明显升高(P<0.01),TRPV5 mRNA与蛋白表达亦明显上调(P<0.01)。 结论: 地塞米松对肾钙转运过程产生抑制作用,是其诱发GIO的重要病理机制之一,鹿茸健骨滴丸通过上调肾组织TRPV5 mRNA与蛋白表达而促进肾钙重吸收,达到治疗GIO的作用。

Effects of Lurong Jiangu Dripping Pills on Expression of TRPV5 in Kidney of Rats with Osteoporosis

Objective: To observe the effect of Lurong Jiangu dripping pills on the bone mineral density (BMD) of femur and expression of TRPV5 in kidney of rats with glucocorticoid-induced osteoporosis (GIO), explore the mechanism of GIO. Method: Eighty-two Wistar rats were randomly divided into normal group(A),model group(B),Lurong Jiangu dripping pills group(C) and Gushukang group(D). By intramuscular injection of dexamethasone(2.5 mg·kg^-1)twice a week rat models was established. The treatment lasted for 9 weeks BMD of femur in vitro was detected with dual-energy X-ray absorptiometry, the mRNA and protein expression of TRPV5 in kidney were detected by real-time quantitative RT-PCR and Western blot methods. Result: Compared with the normal group, the BMD of model group rats decreased evidently(P<0.01), mRNA and protein expression of TRPV5 in kidney of model group rats decreased(P<0.01). Compared with the model group, the BMD of Lurong Jiangu dripping pills group increased(P<0.01), mRNA and protein expression of TRPV5 also increased(P<0.01). Conclusion: The depressant effect of dexamethasone for the calcium transport in kidney was the main pathomechanism of GIO.Lurong Jiangu dripping pills can upregulate the mRNA and protein expression of TRPV5 in kidney to promote renal calcium reabsorption.