石棉表面改性对石棉所致人胚肺细胞毒性及超微结构改变的影响

目的研究温石棉及用柠檬酸铝、混合稀土或亚硒酸钠预处理的温石棉对人胚肺（HEL）细胞存活率及超微结构的影响。方法用不同浓度的柠檬酸铝、混合稀土或亚硒酸钠溶液浸泡温石棉1h后，再将其与HEL细胞共同孵育，测定细胞存活率及观察细胞超微结构。结果不同浓度的温石棉染毒4h或24h，均可使人胚肺细胞的存活率降低，而且其降低程度随石棉浓度的增加而增大，呈一定的剂量-效应关系。经三种化合物预处理的温石棉组HEL细胞存活率高于未处理温石棉组，但差异无统计学意义。从细胞超微结构的改变看，经三种化合物处理的石棉组均轻于单纯的石棉染毒组。结论温石棉染毒可使人胚肺细胞的存活率降低，致使细胞超微结构发生改变，但用三种化合物预处理温石棉，均可减轻温石棉的细胞毒性。

Effects of Surface Modification on Changes of Chrysotile-induced Cytotoxicity and Ultrastructure in Human Embryo Lung Cells

Objective To study the effects of native chrysotile and compounds-pretreated chrysotile on the cytotoxicity and ultrastructure in human embryo lung （HEL） cells. Methods After exposed to native chrysotile or compoundspretreated chrysotile at different doses for different time, the viability of HEL cells was determined by MTF assay and the uhrastructure of HEL cells was observed by electronic microscope. Results When the HEL cells were exposed to native chrysotile at 10, 20, 40, 80, 160μg/ml respectively for 4h, and at 0.625, 1.25, 2.5, 5.0, 10μg/ml respectively for 24h, the viability of HEL cells was decreased significantly compared with control, and showed a dose-effect relationship. But less decrease could be found when cells were exposed to compounds-pretreated chrysotile. While the HEL cells exposed to native chrysotile showed heavy damage in uhrastructure, the HEL cells exposed to compounds-pretreated chrysotile showed less damage of uhrastructure. Conclusion Chrysotile can induced changes of the cytotoxicity and uhrastructure in HEL Cells and pretreatment of chrysotile with aluminium citrate, mixed rare earths or sodium selenite solutions can reduce chrysotile-induced changes of the cytotoxicity and uhrastructure in HEL Cells.