��������������𤸽΢������𤸽�Լ����˹�θҺ������������

In Vitro Adhesion and Retention in Artificial Gastric Acid Fluid of Panax notoginseng Saponins Bio-Adhesive Pellets

??OBJECTIVE To explore the effects of different bio-adhesive materials on in vitro adhesion and retention in artificial gastric acid fluid of Panax notoginseng saponins(PNS) bio-adhesive pellets.METHODS PNS bio-adhesive pellets were prepared with different bio-adhesive materials.The in vitro adhesion of PNS bio-adhesive pellets was determined by the tissue retention method, using HPLC method to determine the contents of PNS(including notoginseng saponins R₁, ginseng saponin Rg1 and ginseng saponin Rb₁) retained in the pellets after a certain period of time in artificial gastric acid fluid.RESULTS The contents of PNS retained in PNS bio-adhesive pellets prepared with chitosan and chitosan-carbomer after being placed in artificial gastric acid fluid for 2 h were the largest, which were (27.60??7.45)% and (19.80??3.55)% for R1, (27.01??5.49)% and (19.67??1.39)% for Rg₁ and (47.07??6.26)% and (51.08??7.44)% for Rb₁, respectively.For in vitro adhesion, HPMC and HPMC-carbomer combination was the best, followed by chitosan and chitosan-carbomer combination.CONCLUSION PNS bio-adhesive pellets prepared with chitosan and chitosan-carbomer combination bio-adhesive materials can protect PNS from degradation in artificial gastric acid fluid and have good adhesion property.